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DOI: 10.1055/s-0030-1252046
© Georg Thieme Verlag KG Stuttgart · New York
Technische Aspekte in regenerativer Medizin und Tissue Engineering von hyalinem Gelenkknorpel
Technological Aspects of Regenerative Medicine and Tissue Engineering of Articular CartilagePublikationsverlauf
eingereicht 08.11.2009
akzeptiert 15.3.2010
Publikationsdatum:
17. Mai 2010 (online)

Zusammenfassung
Tissue Engineering von Gelenkknorpel-Strukturaten wird ein zentrales Problem chirurgisch-orthopädischer Behandlung von sport- und traumaassoziierten Gelenkflächendefekten an großen Gelenken lösen. Es lassen sich komplette biologische osteochondrale Implantate in vitro aus körpereigenem Zellpool erzeugen. Als nativ ausschließlich diffusionsernährtes Gewebe mit nur einem differenzierten Zelltyp eignet sich Gelenkknorpel zudem besonders gut für den innovativen Ansatz. Neben zell- und molekularbiologischen Kompetenzen sind bei diesem biologisch medizinischen Projekt besonders ingenieurstechnische Lösungen der Zellkulturtechnik notwendig. Anhand unseres Projektes zur in vitro Generation osteochondraler Konstrukte zeigen wir Möglichkeiten und Notwendigkeit der Nutzung von Bioreaktoren zur Modifikation der Zellkulturbedingungen und der mechanischen Konditionierung von Knorpelgewebe für funktionstüchtige Implante.
Abstract
The main problem in the treatment of orthopaedic joint-surface defects will be solved by tissue enginering of cartilage implants. Entire biological osteochondral implants can be grown from autologous cells of the patient. The nutrition of articular cartilage is by diffusion only. Therefore the chondrocyte as the unique cell type is perfectly dedicated to the tissue culture approach. Engineering techniques of bioreactors are prerequisite for these biological and medical solutions. With our tissue engineering project for the generation of osteochondral constructs we demonstrate possibilities and characteristics of bioreactors for the modification of cell culture techniques and mechanical conditioning of cartilage tissue for fully operable implants.
Schlüsselwörter
Gelenkknorpel - Bioreaktor - experimentelle Chirurgie
Key words
articular cartilage - bioreactor - tissue engineering
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Korrespondenzadresse
Prof. Norbert M. Meenen
Altonaer Kinderkrankenhaus
Pädiatrische Sportmedizin
Bleickenallee 38
22763 Hamburg
eMail: meenen@uke.uni-hamburg.de