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Am J Perinatol 2010; 27(9): 743-748
DOI: 10.1055/s-0030-1253558
© Thieme Medical Publishers

Treatment with Neuropeptides Attenuates c-fos Expression in a Mouse Model of Fetal Alcohol Syndrome

Maddalena Incerti1 , 2 , Joy Vink1 , 3 , Robin Roberson1 , Daniel Abebe1 , Catherine Y. Spong1
  • 1Unit on Perinatal and Developmental Neurobiology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institute of Health, Bethesda, Maryland
  • 2University of Milano-Bicocca, Department of Obstetrics and Gynecology, Monza, Italy
  • 3Columbia University Medical Center, Division of Maternal Fetal Medicine, New York, New York
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Publication History

Publication Date:
04 May 2010 (online)

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ABSTRACT

Fetal alcohol syndrome (FAS) is the most common nongenetic cause of mental retardation and is characterized by neurodevelopmental anomalies. C-fos is a cellular marker of transcriptional activity in the stress-signal pathway. Previously, we showed the treatment with NAP (NAPVSIPQ) + SAL (SALLRSIPA) reversed the learning deficit after prenatal alcohol exposure in FAS. Our objective was to evaluate if the mechanism of actions of NAP + SAL involves the stress-signal pathway differentiating c-fos expression in mouse brains after prenatal alcohol exposure. C57Bl6/J mice were treated with alcohol (0.03 mL/g) or placebo on gestational day 8. On postnatal day 40, in utero alcohol-exposed males were treated via gavage with 40 μg D-NAP and 40 μg D-SAL (n = 6) or placebo (n = 4); controls were gavaged with placebo daily (n = 12). After learning evaluation, hippocampus, cerebellum, and cortex were isolated. Calibrator-normalized relative real-time polymerase chain reaction and Western blot analysis were performed. Statistics included analysis of variance and post hoc Fisher analysis. Adult treatment with NAP + SAL restored the down-regulation of c-fos in the hippocampus after prenatal alcohol exposure (p < 0.05), but not in the cerebellum. There was no difference in c-fos expression in the cortex. Adult treatment with NAP + SAL restored the down-regulation of c-fos expression in hippocampus attenuating the alcohol-induced alteration of the stress-signal pathway.

KEYWORDS

Fetal alcohol syndrome (FAS) - c-fos - oxidative stress - NAPVSIPQ (NAP) - SALLRSIPA (SAL)

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Maddalena IncertiM.D. 

Unit on Perinatal and Developmental Neurobiology, Eunice Kennedy Shriver NICHD, NIH

Bldg 9/Room 1W125, 9 Memorial Drive, Bethesda, MD 20892-0925

Email: maddalena.incerti@gmail.com