Artificial Ribozyme-Based
Regulators of Gene Expression

Isabelle T. Seemann; Jörg S. Hartig

doi:10.1055/s-0030-1260583

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Synlett 2011(11): 1486-1494
DOI: 10.1055/s-0030-1260583

ACCOUNT

Artificial Ribozyme-Based Regulators of Gene Expression

Isabelle T. Seemann, Jörg S. Hartig*
Department of Chemistry and Konstanz Research School Chemical Biology (KoRS-CB), University of Konstanz, 78457 Konstanz, Germany
Fax: +49(7531)885140; e-Mail: joerg.hartig@uni-konstanz.de;

Further Information

Publication History

Received 23 March 2011
Publication Date:
26 May 2011 (online)

Abstract
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Abstract

The development of RNA-based switches of gene expression is summarized. Most switches are based on the Schistosoma mansonii hammerhead ribozyme (HHR), a self-cleaving RNA sequence that is inserted into an mRNA. Control of HHR self-cleavage is achieved by the attachment of an aptamer domain to the HHR scaffold. External addition of a small-molecule ligand regulates catalytic activity of the ribozyme and hence gene expression. These so-called aptazymes are suited to control several classes of RNA. In addition to mRNA, we have incorporated artificial RNA switches into tRNA as well as the bacterial 16S rRNA. In addition, the presented switches should be widely applicable as demonstrated by experiments in bacteria, yeast, and mammals.

1 Introduction

2 Ribozymes in Nature

3 Ribozymes as Parts for Artificial mRNA Switches

4 Regulation of Further RNA Classes

5 Conclusion

Key words

ribonucleic acid - aptamer - riboswitch - hammerhead - RNA-ligand interaction

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