Modern Determination of Complement Activation

ABSTRACT

Modern, that is, accurate determination of complement activation in vivo requires the simultaneous determination of both naive and activated complement proteins. This is best achieved by the use of native-restricted and neoepitope-specific monoclonal antibodies in sensitive enzyme-linked immunosorbent assays. Several confounding factors also have to be taken into account to allow a correct interpretation of the results.

KEYWORDS

Complement activation - quantitation - ELISA - monoclonal antibodies - neoepitope - native restricted

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