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Semin Thromb Hemost 2010; 36(8): 824-832
DOI: 10.1055/s-0030-1267036
© Thieme Medical Publishers

Microparticle Sizing and Counting Using Light Scattering Methods

Don A. Gabriel1 , 2 , Karen Giordano1
  • 1University of North Carolina School of Medicine, Chapel Hill, North Carolina
  • 2Invitrox, Inc., Durham, North Carolina
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Publication History

Publication Date:
03 November 2010 (online)

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ABSTRACT

New light scattering methods offer many advantages to particle size distribution characterization. In addition to ease of operation, speed, and accuracy, the particle size, particle surface characteristics, interaction of the surface with specific ligands, and hydrodynamic volume of the particle are easily obtained. Extensions of these methods also permit the assessment of surface reactions in real time and without reporter group conjugation to the reactant. These methods offer the ability to examine binding constants and kinetics of binding without chemical modification and offer true advantages in product development and clinical diagnostics and therapeutic monitoring.

KEYWORDS

Quasi elastic light scattering - Mie scattering - electrophoresis - microparticles - particle sizing

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Don A GabrielM.D. Ph.D. 

Division of Hem/Onc, BMT Program, POB – 3rd Floor

UNC School of Medicine, Chapel Hill, NC 27599

Email: dgabriel@invitrox.com