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DOI: 10.1055/s-0030-1269846
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York
Skeletal Muscle Insulin Resistance in Morbid Obesity: The Role of Interleukin-6 and Leptin
Publikationsverlauf
received 29.06.2010
first decision 09.11.2010
accepted 16.11.2010
Publikationsdatum:
02. August 2011 (online)
![](https://www.thieme-connect.de/media/eced/201108/lookinside/thumbnails/10.1055-s-0030-1269846-1.jpg)
Abstract
Background: Although insulin resistance in obesity is established, the link between excess body fat and skeletal muscle insulin resistance is obscure. The aim of this study was to investigate whether cytokines secreted from the subcutaneous adipose tissue are related to the sensitivity of glucose metabolism to insulin in skeletal muscle.
Methods: A meal was given to 14 obese and 10 non-obese women. Plasma samples were taken for 360 min from a forearm vein and from the radial artery for glucose and insulin measurements. Interleukin-6, leptin, TNFα, resistin and adiponectin were measured preprandially from the radial artery and from the superficial epigastric vein. Forearm blood flow was measured with plethysmography.
Results: (1) In obese vs non-obese: (a) Glucose uptake by skeletal muscle was decreased (AUC0–360369±55 vs. 877±146 μmol/100 g tissue, p=0.001) (b) arterial interleukin-6 (2.5±0.5 vs. 1±0.1 pg/ml, p=0.013) and subcutaneous venous interleukin-6 (5±0.5 vs. 3.4±0.5 pg/ml, p=0.027) were increased (c) arterial leptin (63±7 vs. 5±0.6 ng/ml, p<0.0001) and subcutaneous venous leptin 80±8 vs. 6.5±0.7 ng/ml, p<0.0001) were increased. (2) Arterial interleukin-6 (p=0.002) and subcutaneous venous interleukin-6 (p=0.014) were negatively associated with forearm glucose uptake in obese. (3) No association was found between leptin and forearm glucose uptake, after correcting with fat mass.
Conclusions: In morbid obesity: (1) Subcutaneous adipose tissue releases interleukin-6 which could then mediate insulin resistance in skeletal muscle. (2) Although there is increased secretion of leptin by the subcutaneous adipose tissue, leptin levels are not correlated to the sensitivity of glucose metabolism to insulin in muscle.
Key words
adipocytokines - leptin - obesity
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Correspondence
G. DimitriadisMD, DPhil
2nd Department of Internal
Medicine
Research Institute and Diabetes
Center
Athens University, “Attikon”
University Hospital
1 Rimini Street
GR-12462 Haidari
Greece
Telefon: +30/210/583 2547
Fax: +30/210/583 2561
eMail: gdimi@ath.forthnet.gr
eMail: gdimitr@med.uoa.gr