Horm Metab Res 2011; 43(3): 183-187
DOI: 10.1055/s-0030-1270527
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Menin Interacts with β-Catenin in Osteoblast Differentiation

Y. Inoue1 , G. N. Hendy2 , L. Canaff2 , S. Seino1 , 3 , H. Kaji1 , 3
  • 1Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Japan
  • 2Departments of Medicine, Physiology and Human Genetics, McGill University, Montreal, Canada
  • 3Division of Cellular and Molecular Medicine, Department of Physiology and Cellular Biology, Kobe University Graduate School of Medicine, Kobe, Japan
Weitere Informationen

Publikationsverlauf

received 06.09.2010

accepted 20.12.2010

Publikationsdatum:
24. Januar 2011 (online)

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Abstract

Menin promotes the commitment of pluripotent mesenchymal stem cells to the osteoblast lineage by interacting with the BMP-2 signaling molecules Smad1/5, and Runx2. However, the relationship between menin and the Wnt-β-catenin pathway in bone is unclear. Reduction of menin expression by transfection of a menin antisense construct did not alter the levels of β-catenin in mouse mesenchymal C2C12 and osteoblastic MC3T3-E1 cells. However, menin co-immunoprecipitated with β-catenin as well as LEF-1 in C2C12 and MC3T3-E1 cells. Reduction of menin expression by antisense menin transfection antagonized β-catenin-induced transcriptional activity of the pGL3-OT luciferase reporter construct in C2C12 and MC3T3-E1 cells. Antisense menin transfection antagonized the BMP-2 and β-catenin-stimulated increases in Runx2 and alkaline phosphatase levels in C2C12 cells. The data show that menin interacts with β-catenin in mouse mesenchymal and osteoblastic cells, and suggest that the interaction is important for osteoblast differentiation.