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DOI: 10.1055/s-0030-1270527
© Georg Thieme Verlag KG Stuttgart · New York
Menin Interacts with β-Catenin in Osteoblast Differentiation
Publication History
received 06.09.2010
accepted 20.12.2010
Publication Date:
24 January 2011 (online)
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Abstract
Menin promotes the commitment of pluripotent mesenchymal stem cells to the osteoblast lineage by interacting with the BMP-2 signaling molecules Smad1/5, and Runx2. However, the relationship between menin and the Wnt-β-catenin pathway in bone is unclear. Reduction of menin expression by transfection of a menin antisense construct did not alter the levels of β-catenin in mouse mesenchymal C2C12 and osteoblastic MC3T3-E1 cells. However, menin co-immunoprecipitated with β-catenin as well as LEF-1 in C2C12 and MC3T3-E1 cells. Reduction of menin expression by antisense menin transfection antagonized β-catenin-induced transcriptional activity of the pGL3-OT luciferase reporter construct in C2C12 and MC3T3-E1 cells. Antisense menin transfection antagonized the BMP-2 and β-catenin-stimulated increases in Runx2 and alkaline phosphatase levels in C2C12 cells. The data show that menin interacts with β-catenin in mouse mesenchymal and osteoblastic cells, and suggest that the interaction is important for osteoblast differentiation.
Key words
menin - β-catenin - osteoblast
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Correspondence
H. Kaji
Division of Diabetes,
Metabolism and Endocrinology
Department of Internal
Medicine and Division of
Cellular and Molecular Medicine
Kobe University Graduate
School of Medicine
7-5-2 Kusunoki-cho
650-0017 Chuo-ku, Kobe
Japan
Phone: +81/78/382 5861
Fax: +81/78/382 2080
Email: hiroshik@med.kobe-u.ac.jp