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Planta Med 2011; 77(11): 1139-1148
DOI: 10.1055/s-0030-1270719
Lectures GA Conference
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Herbal Medicines and Infectious Diseases: Characterization by LC-SPE-NMR of Some Medicinal Plant Extracts Used against Malaria

Yong-Jiang Xu1 , Rica Capistrano1  I. , Liene Dhooghe1 , Kenn Foubert1 , Filip Lemière2 , Sheila Maregesi3 , Aliou Baldé4 , Sandra Apers1 , Luc Pieters1
  • 1Laboratory of Pharmacognosy and Pharmaceutical Analysis, Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium
  • 2Nucleoside Research and Mass Spectrometry Unit, Department of Chemistry, Antwerp, Belgium
  • 3Department of Pharmacognosy, School of Pharmacy, Muhimbili University of Health and Allied Sciences (MUHAS), Dar-Es-Salaam, Tanzania (present address)
  • 4Département de Pharmacie, Faculté de Médecine, Pharmacie et Odontostomatologie, Université de Conakry; Centre de Recherche et de Valorisation des Plantes Médicinales de Dubréka, Conakry, Guinea
Further Information

Publication History

received October 19, 2010 revised Dec. 1, 2010

accepted January 1, 2011

Publication Date:
15 February 2011 (online)

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Abstract

The extracts of two medicinal plants used in traditional medicine against malaria were characterized by means of an LC-SPE-NMR and LC-MS platform. The structure of a series of major constituents from Bafodeya benna, as well as minor constituents from Ormocarpum kirkii, was determined. Bafodeya benna was found to contain (2R,3R)-taxifolin-3-O-α-L-rhamnoside or astilbin, and its isomers neoastilbin, neoisoastilbin, and isoastilbin, as well as quercetin-3-O-α-L-rhamnoside. From Ormocarpum kirkii, a series of known flavonoids and biflavonoids was obtained, as well as three new compounds, i.e., 7,7′′-di-O-β-D-glucosyl-(−)-chamaejasmin, 7-O-β-D-glucosyl-(I-3,II-3)-biliquiritigenin, and isovitexin-(I-3,II-3)-naringenin. The isolated constituents may explain, at least in part, the traditional use against malaria. LC-SPE-NMR, in combination with LC-MS, is a powerful tool for the fast characterization of plant extracts, in order to define priorities at an early stage of a fractionation procedure. In addition, herbal medicinal products can completely be characterized, both with regard to their major as well as their minor constituents.

Key words

LC-SPE-NMR - malaria - Bafodeya benna - Chrysobalanaceae - Ormocarpum kirkii - Papilionaceae

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Prof. Dr. Luc Pieters

Laboratory of Pharmacognosy and Pharmaceutical Analysis
Department of Pharmaceutical Sciences
University of Antwerp

Universiteitsplein 1

2610 Antwerp

Belgium

Phone: +32 32 65 27 15

Fax: +32 32 65 27 09

Email: luc.pieters@ua.ac.be

    www.thieme-connect.de/ejournals/toc/plantamedica