Profiling of Phenolics from Tephrosia cinerea

Abstract

An HPLC-ESIMSⁿ method, based on high-performance liquid chromatography coupled to electrospray positive ionization multistage ion trap mass spectrometry, has been used to rapidly identify and guide the isolation of phenolic constituents from the methanol extract of the aerial parts of T. cinerea. On the basis of the results of the online screening by HPLC-ESIMSⁿ, 17 phenolic constituents, including two new compounds, namely demethylapollinin 7-O-β-D-glucopyranoside (1) and cineroside A (17), were isolated, and their structures were unambiguously assigned by the extensive use of 1D- and 2D-NMR experiments.

Key words

Tephrosia cinerea - Fabaceae - phenolic constituents - LC-MS profile

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