Planta Med 2011; 77(16): 1855-1860
DOI: 10.1055/s-0030-1271200
Analytical Studies
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Comparative Analyses of Chromatographic Fingerprints of the Roots of Polygonum multiflorum Thunb. and Their Processed Products Using RRLC/DAD/ESI-MSn

Zhenli Liu1 , Yuanyan Liu2 , Chao Wang1 , Na Guo3 , Zhiqian Song1 , Chun Wang1 , Lei Xia1 , Aiping Lu4
  • 1Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China
  • 2School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China
  • 3Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China
  • 4Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, China
Further Information

Publication History

received March 27, 2011 revised April 21, 2011

accepted May 12, 2011

Publication Date:
14 June 2011 (online)

Abstract

The dried roots of Polygonum multiflorum Thunb. (Heshouwu) and their processed products (Zhi-heshouwu) are widely used in traditional Chinese medicine, yet their therapeutic effects are different. Previous investigations focused mainly on the differences between Heshouwu and Zhi-heshouwu in the contents of several known compounds. In this study, a rapid resolution liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (RRLC/DAD/ESI-MSn) method was developed for the comparative analysis of the components of Heshouwu and Zhi-heshouwu. A total of 23 compounds were identified or tentatively characterized. We found that 16 batches of Heshouwu and 15 batches of Zhi-heshouwu samples shared eight compounds, including gallic acid; 3,5,4′-tetrahydroxylstilbene-2,3-di-O-glucoside, cis-2,3,5,4′-tetrahydroxylstilbene-2-O-β-D-glucoside, trans-2,3,5,4′-tetrahydroxylstilbene-2-O-β-D-glucoside, emodin-8-O-β-D-glucoside, physcion-8-O-β-D-glucoside, emodin, and physcion. Nevertheless, the relative amounts of gallic acid, emodin, and physcion were very high in Zhi-heshouwu samples compared to those in Heshouwu samples. Six compounds disappeared after processing and were unique for Heshouwu: catechin, flavanol gallate dimer, polygonimitin B, emodin-1-O-glucoside, emodin-8-O-(6′-O-malonyl)-glucoside, and physcion-8-O-(6′-O-malonyl)-glucoside. Three compounds were unique for Zhi-heshouwu: hydroxymaltol, 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one, and 5-hydroxymethyl furfural. These results suggest that the types and relative amounts of the chemical components of Heshouwu and Zhi-heshouwu are different.

Supporting Information

References

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Prof. Aiping Lu

Institute of Basic Research in Clinical Medicine
China Academy of Chinese Medical Sciences

Nanxiaojie 18, Dongzhimennei

Beijing 100700

China

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