Planta Med 2011; 77 - P_2
DOI: 10.1055/s-0031-1273531

Molecular Analysis of Genetic Stability in Synthetic Seeds Grown Cannabis sativa L. Plants

H Lata 1, S Chandra 1, N Techen 1, IA Khan 1, 2, 3, MA ElSohly 1, 4
  • 1National Center for Natural Product Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, MS 38677, USA
  • 2Department of Pharmacognosy, School of Pharmacy, University of Mississippi, MS 38677, USA
  • 3Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
  • 4Department of Pharmaceutics, School of Pharmacy, University of Mississippi, University, MS 38677, USA

The increasing utilization of synthetic seeds for germplasm conservation and propagation necessitates the assessment of genetic stability of conserved propagules following their plantlet conversion. The present study was undertaken to assess the genetic stability of synthetic seeds grown Cannabis sativa L. plants during in vitro multiplication and storage at different growth conditions, using Inter Simple Sequence Repeat (ISSR) DNA fingerprinting. Molecular analysis of randomly selected plants from each batch was conducted using 14 ISSR markers. Of the 14 primers tested, 8 produced amplification products. All the ISSR profiles from in vitro stored plants were monomorphic and comparable to mother plant which confirms the genetic stability among clones and mother plant.

Acknowledgement: This work was supported in part by the National Institute on Drug Abuse (NIDA), National Institute of Health (NIH), Department of Health and Human Services, USA, Contract No. N01DA-10–7773.