Planta Med 2011; 77 - P_56
DOI: 10.1055/s-0031-1273585

Simultaneous Determination of 10 Bioactive Compounds from the Roots of Cynanchum paniculatum by using HPLC-DAD

JB Weon 1, HJ Yang 1, BH Lee 1, CJ Ma 1, 2
  • 1Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200–701, Korea
  • 2Research Institute of Biotechnology, Kangwon National University, Chuncheon 200–701, Korea

A novel and reliable high performance liquid chromatography coupled with diode array detector method has been established, for simultaneous determination of 10 bioactive compounds isolated from Cynanchum paniculatum Kitagawa, one of the herbal medicines. The chromatography analysis was performed on a SHISEIDO C18 column (S-5µm, 4.6mm I.D.×250mm) at 35°C with a gradient elution of acetonitrile and water at a flow rate of 1ml/min, and UV detection at 210, 230 and 280nm. The method was validated for linearity, precision and accuracy. All calibration curves showed good linear regression (r 2 >0.9996). Limits of detection (LOD) and limits of quantification (LOQ) fell in the ranges 0.01–0.28µg/ml and 0.04–0.83µg/ml, respectively. The relative standard deviation (RSD) of the intra- and inter- day test, precision test were within 1.92% and 2.43%, respectively. The mean recovery of all ranged from 92.82 to 107.96% with RSD values 0.12–2.18%. The results of validation appeared that this established method was very accurate and stabilized for the quantification of 10 bioactive compounds isolated from C. paniculatum.