Planta Med 2011; 77 - P_79
DOI: 10.1055/s-0031-1273608

Chemical Analysis of Lancea tibetica using UPLC-UV/MS and ESI-MSn Methods

ZH Song 1, 2, YH Wang 2, B Avula 2, TJ Smillie 2, ZZ Qian 1, IA Khan 2, 3, 4
  • 1Chinese Pharmacopeia Commission, Beijing, 100061, P. R. China
  • 2National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, MS 38677, USA
  • 3Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA
  • 4Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia

Lancea tibetica Hook. F. et Thoms (Family: Scrophulariaceae) is a Tibetan medicinal plant used for the prevention and treatment of leukemia, heart disease and cough in traditional Chinese medicine. The pharmacological and antioxidant effects of L. tibetica are mainly attributed to the content of phenylpropanoid glycosides and lignans [1,2]. As part of a program to address the issues of authenticity of material source, safety, quality assurance, and quality control of botanical and dietary supplements, nine furofurano lignans along with verbascoside, isoverbascoside, β-sitosterol and oleanolic acid were identified from L. tibetica in our research group. In the present work, both UPLC-UV/MS and ESI-MS/MS methods were applied for the quantitative and qualitative determination of lignans, verbascoside and isobascoside in L. tibetica. The reference standards include verbascoside (1), isoverbascoside (2), lantibeside (3), phillyrin (4), lantibeside B (5), lantibeside C (6), tibeticoside A (7), styraxjaponoside C (8), sylvatesmin (9), (+)-piperitol (10) and horsfieldin (11). The separation of standard compounds was achieved by using C-18 column material in UPLC method coupled with PDA and SQD detectors; a mixture of water and acetonitrile, both containing 0.05% formic acid, has been selected as the mobile phase. The column temperature was maintained at 40°C. Structures of reference standards were further characterized by ESI-MS/MS method, and discussed the proposed fragmentation pathway. The developed UPLC-UV/MS and MS/MS methods will be useful for the determination of lignans, verbascoside and isobascoside in L. tibetica, and in future biological studies related to L. tibetica.

Figure 1: UPLC-UV chromatograms of mixture of standards (A) and L. tibetica (B-C) at 280nm

Figure 2: Proposed Fragmentation Pathway of Lantibeside B under ESI-MS Negative Mode

Acknowledgements: This research is supported in part by „Science Based Authentication of Dietary Supplements“ and „Botanical Dietary Supplement Research“ funded by the Food and Drug Administration grant numbers 5U01FD002071–10 and 1U01FD003871–02, and the United States Department of Agriculture, Agricultural Research Service, Specific Cooperative Agreement No. 58–6408–2-0009.

References: [1] Chinese Herbs Editor, (2002) Zhonghua Bencao (Volume of Tibetan Medicine), Shanghai Science and Technology Press (China), p.192. [2] Li J, et al. (1997) Planta Medica, 63: 499–501.