Element and redox homeostasis in rats after red wine consumption

In our earlier human study unfourable changes were observed in IL-1alfa, IL-2, VEGF and EGF level in women and TNF-alfa level in men after red wine consumption (women: 2 dl/day for 30 days and men: 3 dl/day for 30 days). Redox parameters changed only a small-scale. The concentration of Ca-, Pb- and Sr in erytrocyte decreased significantly in the case of both sexes, while the depletion of Mg and Zn was found in women. The aim of this work was to determine the changes of redox and element homeostasis after red wine consumption in rats and to prove the negative effects found in human study.

Male Wistar rats (n=5–5; 150–200g bw) were treated with red wine (Egri Cuvée, alcohol content: 10.5%, resveratrol content: 12.03mg/ml, dose: 8ml/body weight kg) and with alcohol (aqueous solution, alcohol content equal to red wine) for 10 days. Rutin laboratory parameters were measured in plasma, as well as redox parameters (total scavenger capacity, H-donating activity) and element content were determined in erytrocyte. Rutin laboratory parameters indicated that the liver were not damaged by red wine and alcohol consumption in short rat experiment. The changes of redox parameters were not significant, although some alterations were observed. The concentration of Ba, Ca, Co, Cu, Fe, Li, Mg, Ni, P, Pb, Zn in erytrocyte increased and the concentration of Mn, Si decreased by the red wine and alcohol consumption. The results show that the red wine and alcohol affects the redox and element homeostasis, although longer time is needed for the provement of unfourable effect.

The work was supported by ETT 002/02.