Spirostanol Saponins Derivated from the Seeds of Trigonella foenum-graecum by β-Glucosidase Hydrolysis and Their Inhibitory Effects on Rat Platelet Aggregation

Abstract

Nine spirostanol saponins (1–9) and seven mixtures of 25R and 25S spirostanol saponin isomers (10–16) were obtained from the seeds of Trigonella foenum-graecum after enzymatic hydrolysis of the furostanol saponin fraction by β-glucosidase. Their structures were determined by NMR and MS spectroscopy. Among them, 1-4, 6, 8, and 9 were new compounds and five, 11b, 12a, 13b, 14a, and 14b, were new structures observed from seven mixtures. In addition, the inhibitory effects of all saponins on rat platelet aggregation were evaluated.

Key words

Trigonella foenum-graecum - Leguminosae - spirostanol saponins - enzymatic hydrolysis - inhibitory platelet aggregation activity

References

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1 These authors contributed equally to this work.