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Planta Med 2012; 78(4): 368-376
DOI: 10.1055/s-0031-1280441
Natural Product Chemistry
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Isolation and Bioactivity Evaluation of Terpenoids from the Medicinal Fungus Ganoderma sinense

Jie-Qing Liu1 , Cui-Fang Wang1 , Yan Li 1 , Huai-Rong Luo1 , Ming-Hua Qiu1
  • 1State Key Laboratory of Photochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, P. R. China
Further Information

Publication History

received August 13, 2011 revised Nov. 8, 2011

accepted Nov. 22, 2011

Publication Date:
12 December 2011 (online)

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Abstract

A new pentanorlanostane, ganosineniol A (1), eight new lanostane triterpenoids, ganosinoside A (2), ganoderic acid Jc (3), ganoderic acid Jd (4), ganodermatetraol (5), ganolucidic acid γa (6), ganolucidate F (7), ganoderiol J (8), and methyl lucidenate Ha (9), and a new sesquiterpenoid, ganosinensine (10), together with eleven known triterpenoids (11–21), were isolated from the fruiting bodies of the fungus Ganoderma sinense. Chemical structures were determined based on spectroscopic evidence, including 1D, 2D NMR, and mass spectral data. Furthermore, all isolates were tested for cytotoxic activity and induction ability of hPXR-mediated CYP3A4 expression. Among them, ganoderic acid Jc (3) displayed selective inhibitory activity against HL-60 cells (IC50 = 8.30 µM), and ganoderiol E (11) exhibited selective cytotoxic activity against MCF-7 cells (IC50 = 6.35 µM). Meanwhile, compounds 5, 7, and ganolucidic acids B and C (19, 20) showed induction ability of hPXR-mediated CYP3A4 expression.

Key words

Ganoderma sinense - Ganodermataceae - triterpenoids - sesquiterpene - cytotoxicity - CYP3A4 induction

Supporting Information

References

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Prof. Dr. Ming-Hua Qiu

State Key Laboratory of Photochemistry and Plant Resources in West China
Kunming Institute of Botany
Chinese Academy of Sciences

Lanhei Road, 132

Kunming 650204

Yunnan

P. R. China

Phone: +86 87 15 22 32 57

Fax: +86 87 15 22 32 55

Email: mhchiu@mail.kib.ac.cn