Subscribe to RSS
DOI: 10.1055/s-0031-1280449
© Georg Thieme Verlag KG Stuttgart · New York
Screening the Antiangiogenic Activity of Medicinal Plants Grown and Sold in Jordan
Publication History
received October 10, 2011
revised October 19, 2011
accepted Nov. 24, 2011
Publication Date:
15 December 2011 (online)

Abstract
Angiogenesis is essential for the growth, invasion, and metastasis of most solid tumors and has become a valuable pharmacological target for cancer prevention and treatment. This study was performed to assess the antiangiogenic activity of 31 medicinal plants grown and sold in Jordan. The antiangiogenic activity was assessed using the rat aortic ring assay. Out of 31 extracts, 15 extracts showed more than 50 % inhibition of the blood vessels outgrowth from the primary tissue explants (p = 0.000). Three of these 15 extracts showed a potential cytotoxic effect on normal fibroblast cells. Four extracts shared antiangiogenic and antiproliferative activity towards MCF7 breast cancer cell lines. Eight extracts demonstrated selective antiangiogenic activity. This is the first report demonstrating the potential antiangiogenic activity of Artemisia judaica, Aloysia citriodora, Salvia egyptiaca, and Calendula arvensis. Some extracts with antiangiogenic activity exhibited selectivity against the endothelial cells proliferation, demonstrating a direct inhibitory activity against the key step in tumor angiogenesis.
Key words
anti-angiogenesis - endothelial cells - MCF7 - plant extract - Jordan
References
- 1
Folkman J.
Angiogenesis in cancer, vascular, rheumatic and other disease.
Nat Med.
1995;
1
27-31
MissingFormLabel
- 2
Carmeliet P.
Angiogenesis in health and disease.
Nat Med.
2003;
9
653-660
MissingFormLabel
- 3
Risau W.
Mechanisms of angiogenesis.
Nature.
1997;
386
671-674
MissingFormLabel
- 4
Shoeb M.
Anticancer agents from medicinal plants.
Bangladesh J Pharmacol.
2006;
1
35-41
MissingFormLabel
- 5
Balunas M J, Kinghorn A D.
Drug discovery from medicinal plants.
Life Sci.
2005;
78
431-441
MissingFormLabel
- 6
Wang S, Zheng Z, Weng Y, Yu Y, Zhang D, Fan W, Dai R, Hu Z.
Angiogenesis and anti-angiogenesis activity of Chinese medicinal herbal extracts.
Life Sci.
2004;
74
2467-2478
MissingFormLabel
- 7
Aisha A F A, Abu-Salah K M, Darwis Y, Abdul Majid A M S.
Screening of antiangiogenic activity of some tropical plants by rat aorta ring assay.
Int J Pharmacol.
2009;
5
370-376
MissingFormLabel
- 8
Abu-Dahab R, Afifi F.
Antiproliferative activity of selected medicinal plants of Jordan against a breast
adenocarcinoma cell line (MCF7).
Sci Pharm.
2007;
75
121-136
MissingFormLabel
- 9
Talib W H, Mahasneh A M.
Antiproliferative activity of plant extracts used against cancer in traditional medicine.
Sci Pharm.
2010;
78
33-45
MissingFormLabel
- 10
Al-Kalaldeh J Z, Abu-Dahab R, Afifi F U.
Volatile oil composition and antiproliferative activity of Laurus nobilis, Origanum syriacum, Origanum vulgare, and Salvia triloba against human breast adenocarcinoma cells.
Nutr Res.
2010;
30
271-278
MissingFormLabel
- 11
Dudek A Z, Pawlak W Z, Kirstein M N.
Molecular targets in the inhibition of angiogenesis.
Expert Opin Ther Targets.
2003;
7
527-541
MissingFormLabel
- 12
Naldini A, Carraro F.
Role of inflammatory mediators in angiogenesis.
Curr Drug Targets Inflamm Allergy.
2005;
4
3-8
MissingFormLabel
- 13
Atta A H, Alkofahi A.
Anti-nociceptive and anti-inflammatory effects of some Jordanian medicinal plant extracts.
J Ethnopharmacol.
1998;
60
117-124
MissingFormLabel
- 14
Lenfeld J, Motl O.
Antiinflammatory activity of Conyza canadensis.
Planta Med.
1986;
52
430-431
MissingFormLabel
- 15
Al-Hindawi M K, Al-Deen I H, Nabi M H, Ismail M A.
Anti-inflammatory activity of some Iraqi plants using intact rats.
J Ethnopharmacol.
1989;
26
163-168
MissingFormLabel
- 16
Jin M H, Yook J, Lee E, Lin C X, Quan Z, Son K H, Bae K H, Kim H P, Kang S S, Chang H W.
Anti-inflammatory activity of Ailanthus altissima in ovalbumin-induced lung inflammation.
Biol Pharm Bull.
2006;
29
884-888
MissingFormLabel
- 17
Ozturk B, Apaydin S, Goldeli E, Ince I, Zeybek U.
Hypericum triquetrifolium Turra. extract exhibits antiinflammatory activity in the rat.
J Ethnopharmacol.
2002;
80
207-209
MissingFormLabel
- 18
Rahman A U, Nasim S, Baig I, Jalil S, Orhan I, Sener B, Choudhary M I.
Anti-inflammatory isoflavonoids from the rhizomes of Iris germanica.
J Ethnopharmacol.
2003;
86
177-180
MissingFormLabel
- 19
Rahman A U, Nasim S, Baig I, Jalil S, Orhan I, Sener B, Choudhary M I.
A review of the efficacy of traditional Iranian medicine for inflammatory bowel disease.
World J Gastroenterol.
2010;
16
4504-4514
MissingFormLabel
- 20
El-Shazly A M, Hussein K T.
Chemical analysis and biological activities of the essential oil of Teucrium leucocladum Boiss (Lamiaceae).
Biochem Syst Ecol.
2004;
32
665-674
MissingFormLabel
- 21
Singh J, Singh A J.
Anti-inflammatory and anti-pyretic activity of Vitis vinifera leaves extracts.
The Pharma Res J.
2010;
3
172-182
MissingFormLabel
- 22
Hajhashemi V, Ghannadi A, Sharif B.
Anti-inflammatory and analgesic properties of the leaf extracts and essential oil
of Lavandula angustifolia Mill.
J Ethnopharmacol.
2003;
89
67-71
MissingFormLabel
- 23
Ukiya M, Akihisa T, Yasukawa K, Tokuda H, Suzuki T, Kimura Y.
Anti-inflammatory, anti-tumor-promoting, and cytotoxic activities of constituents
of pot marigold (Calendula officinalis) flowers.
J Nat Prod.
2006;
69
1692-1696
MissingFormLabel
- 24
Loizzo M R, Menichini F, Conforti F, Tundis R, Bonesi M, Saab A M, Statti G A, Cindio B, Houghton P J, Menichini F, Frega N G.
Chemical analysis, antioxidant, antiinflammatory and anticholinesterase activities
of Origanum ehrenbergii Boiss and Origanum syriacum L. essential oils.
Food Chem.
2009;
117
174-180
MissingFormLabel
- 25
Hudaib M, Mohammad M, Bustanji Y, Tayyem R, Yousef M, Abuirjeie M, Aburjai T.
Ethnopharmacological survey of medicinal plants in Jordan, Mujib Nature Reserve and
surrounding area.
J Ethnopharmacol.
2008;
120
63-71
MissingFormLabel
- 26
Ahmed S, Wang N, Hafeez B B, Cheruvu V K, Haqqi T M.
Punica granatum L. extract inhibits IL-1beta-induced expression of matrix metalloproteinases by inhibiting
the activation of MAP kinases and NF-kappaB in human chondrocytes in vitro.
J Nutr.
2005;
135
2096-2102
MissingFormLabel
- 27
Ahmadiani A, Javan M, Semnanian S, Barat E, Kamalinejad M.
Anti-inflammatory and antipyretic effects of Trigonella foenum-graecum leaves extract in the rat.
J Ethnopharmacol.
2001;
75
283-286
MissingFormLabel
- 28
Mueller M, Hobiger S, Jungbauer A.
Anti-inflammatory activity of extracts from fruits, herbs and spices.
Food Chem.
2010;
122
987-996
MissingFormLabel
- 29
Brown K J, Maynes S F, Bezos A, Maguire D J, Ford M D, Parish C R.
A novel in vitro assay for human angiogenesis.
Lab Invest.
1996;
75
539-555
MissingFormLabel
- 30
Nicosia R F, Lin Y J, Hazelton D, Qian X.
Endogenous regulation of angiogenesis in the rat aorta model. Role of vascular endothelial
growth factor.
Am J Pathol.
1997;
151
1379-1386
MissingFormLabel
- 31 Boik J. Natural compounds in cancer therapy. Minnesota: Oregon Medical Press; 2001: 25
MissingFormLabel
- 32
Kwon H K, Jeon W K, Hwang J S, Lee C G, So J S, Park J A, Ko B S, Im S H.
Cinnamon extract suppresses tumor progression by modulating angiogenesis and the effector
function of CD8+ T cells.
Cancer Lett.
2009;
278
174-182
MissingFormLabel
- 33
Aisha A F A, Nassar Z D, Siddiqui M J, Abu-Salah K M, Alrokayan S A, Ismail Z, Abdul Majid A M S.
Evaluation of antiangiogenic, cytotoxic and antioxidant effects of Syzygium aromaticum L. extracts.
Asian J Biol Sci.
2011;
4
282-290
MissingFormLabel
- 34
Jurenka J S.
Therapeutic applications of pomegranate (Punica granatum L.): a review.
Altern Med Rev.
2008;
13
128-144
MissingFormLabel
- 35
Sagar S M, Yance D, Wong R K.
Natural health products that inhibit angiogenesis: a potential source for investigational
new agents to treat cancer-Part 2.
Curr Oncol.
2006;
13
99-107
MissingFormLabel
- 36
Al-Harbi M M, Qureshi S, Raza M, Ahmed M M, Giangreco A B, Shah A H.
Anticarcinogenic effect of Commiphora molmol on solid tumors induced by Ehrlich carcinoma cells in mice.
Chemotherapy.
1994;
40
337-347
MissingFormLabel
Dr. Malek Zihlif
Department of Pharmacology
Faculty of Medicine
University of Jordan
Amman 11942
Jordan
Phone: +96 2 65 35 50 00
Fax: +96 2 65 35 67 46
Email: M.zihlif@ju.edu.jo