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Horm Metab Res 2011; 43(08): 531-536
DOI: 10.1055/s-0031-1280830
Original Basic
Georg Thieme Verlag KG Stuttgart · New York

Reduced Porcine Islet Isolation Yield in the Presence of Hyperemic Islets

D.E. Hilling
1   Department of Surgery Leiden University Medical Center, Leiden, The Netherlands
,
J.K.R.A. Rijkelijkhuizen
1   Department of Surgery Leiden University Medical Center, Leiden, The Netherlands
,
H.A.M. Töns
1   Department of Surgery Leiden University Medical Center, Leiden, The Netherlands
,
P.J. Marang-van de Mheen
2   Department of Medical Decision Making, Leiden University Medical Center, Leiden, The Netherlands
,
J.W.M. Merks
3   IPG, Institute for Pig Genetics B.V., Beuningen, The Netherlands
,
O.T. Terpstra
1   Department of Surgery Leiden University Medical Center, Leiden, The Netherlands
,
E. Bouwman
1   Department of Surgery Leiden University Medical Center, Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

received 11 September 2010

accepted 07 June 2011

Publication Date:
06 July 2011 (online)

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Abstract

When studying histological characteristics of porcine pancreata in relation to islet isolation, a remarkably high number of hyperemic islets (HIs) was encountered. The abnormalities observed in these HIs ranged from a single dilated vessel to hemorrhages extending into the surrounding exocrine tissue. The aim of the present study was to compare pancreata with and without HI on islet isolation outcomes. This study involved a histological examination of 143 purebred (74 juvenile and 69 adult) and 47 crossbred (only juvenile) porcine pancreata. Islet isolation was performed in 48 purebred adult pigs and in 25 crossbred pigs. Tissue samples were stained with Aldehyde Fuchsine. The presence of HIs was scored semi-quantitatively (HI–, HI+). We observed HIs in 48% of the purebred and in 68% of the crossbred pigs. However, only 3.3±3.1% and 3.1±4.7% of all assessed islets was hyperemic in HI+ pancreata in purebred and crossbred pigs, respectively. In both groups, significantly higher endocrine cell mass was found in the HI+ pancreata (p<0.01). When the higher endocrine cell mass was taken into account, we found significantly lower yields in the HI+ pancreata in both purebred and crossbred pigs (p=0.03 in both groups). The presence of HIs occurs frequently in porcine donor-pancreata and is associated with reduced isolation outcomes.

Key words

hyperemic - islet isolation - islet transplantation - porcine - pancreas
 

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