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Horm Metab Res 2011; 43(09): 607-613
DOI: 10.1055/s-0031-1284355
Original Basic
Georg Thieme Verlag KG Stuttgart · New York

Enhanced Oxidative Stress and Platelet Activation Combined with Reduced Antioxidant Capacity in Obese Prepubertal and Adolescent Girls with Full or Partial Metabolic Syndrome

I. Karamouzis
1   Division of Endocrinology, Diabetology and Metabolism, First Department of Pediatrics, Athens University Medical School, “Aghia Sophia” Children’s Hospital, Athens, Greece
,
P. Pervanidou
1   Division of Endocrinology, Diabetology and Metabolism, First Department of Pediatrics, Athens University Medical School, “Aghia Sophia” Children’s Hospital, Athens, Greece
,
R. Berardelli
2   Division of Endocrinology, Diabetology and Metabolism, Department of Internal Medicine, University of Turin, Italy
,
S. Iliadis
3   Laboratory of Biological Chemistry, Faculty of Medicine, Aristotle University of Thessaloniki, Greece
,
I. Papassotiriou
4   Department of Clinical Biochemistry, “Aghia Sophia” Children’s Hospital, Athens, Greece
,
M. Karamouzis
3   Laboratory of Biological Chemistry, Faculty of Medicine, Aristotle University of Thessaloniki, Greece
,
G. P. Chrousos
1   Division of Endocrinology, Diabetology and Metabolism, First Department of Pediatrics, Athens University Medical School, “Aghia Sophia” Children’s Hospital, Athens, Greece
,
C. Kanaka-Gantenbein
1   Division of Endocrinology, Diabetology and Metabolism, First Department of Pediatrics, Athens University Medical School, “Aghia Sophia” Children’s Hospital, Athens, Greece
› Author Affiliations
Further Information

Publication History

received 11 January 2011

accepted 30 June 2011

Publication Date:
05 August 2011 (online)

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Abstract

In adults, obesity is a main factor implicated in increased oxidative stress (OS), platelet activation (PA) and impaired antioxidant status (AS), all predisposing factors for cardiovascular disease leading to increased morbidity and mortality. Furthermore, the metabolic syndrome (MetS) is an important cardiovascular risk factor, which progressively develops and may already be present during late childhood or adolescence. However, scarce data exist on oxidative-antioxidant balance and PA in childhood and adolescence in the presence of partial (PMetS) or full MetS. The aim of the study was to evaluate OS, PA, and AS in prepubertal and adolescent obese girls with partial or full MetS. 96 girls with a clinical and metabolic evaluation for obesity and 44 healthy normal-weight sex- and age-matched girls were studied. IDF-adopted criteria were used to define full and partial MetS and the patient population was divided into 4 groups: the first comprised 31 pre-pubertal girls with PMetS (PR-PMetS), the second 37 adolescents with PMetS (AD-PMetS), the third 10 prepubertal girls with full MetS (PR-MetS), and the fourth 18 adolescents with full MetS (AD-MetS). The OS was evaluated by measuring plasma 15-F2t-Isoprostane levels (15-F2t-IsoP) and protein carbonyls, PA by thromboxane B2 levels (TXB2), and AS by serum vitamin E and plasma total antioxidant capacity (TAC) levels. 15-F2t-IsoP, protein carbonyls, and TXB2 levels were significantly gradually amplified, and vitamin E and TAC reduced, and significantly correlated with obesity from childhood to adolescence and from partial to full MetS. This study demonstrates the loss of the normal homeostatic balance between oxidant-antioxidant state in obese children and adolescents with manifestations of partial and full MetS.

Key words

antioxidant state - isoprostane - thromboxane - pediatric obesity - carbonyl proteins
 

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