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Thorac Cardiovasc Surg 2012; 60(01): 005-010
DOI: 10.1055/s-0031-1293607
Original Basic Science
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

The Protective Effect of Adrenomedullin on Renal Injury, in a Model of Abdominal Aorta Cross-Clamping

Eser Öz Oyar
1   Department of Physiology, Faculty of Medicine, Gazi University, Ankara, Turkey
,
İlker Kiriş
2   Department of Cardiovascular Surgery, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey
,
Şenol Gülmen
2   Department of Cardiovascular Surgery, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey
,
Betül Mermi Ceyhan
3   Department of Biochemistry, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey
,
Medine Cumhur Cüre
3   Department of Biochemistry, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey
,
Namık Delibaş
3   Department of Biochemistry, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey
,
Neşe Lortlar
4   Department of Medical Histology and Embryology, Faculty of Medicine, Gazi University, Ankara, Turkey
,
Hüseyin Okutan
2   Department of Cardiovascular Surgery, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey
› Author Affiliations
Further Information

Publication History

21 December 2010

09 June 2011

Publication Date:
05 January 2012 (online)

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Abstract

Renal injury induced by aortic ischemia–reperfusion (IR) is an important factor in the development of postoperative acute renal failure following abdominal aortic surgery. The aim of this study was to examine the effect of adrenomedullin (AM) on kidney injury induced by infrarenal abdominal aortic IR in rats. Thirty-two Wistar Albino rats were randomized into four groups (eight per group) as follows: Control group, IR group (120-minute ischemia and 120-minute reperfusion), IR + AM group (a bolus intravenously of 0.05 µg/kg/min AM), and control + AM group. At the end of the experiment, blood and kidney tissue specimens were obtained for biochemical analysis. Immunohistological evaluation of the rat kidney tissues was also done. IR significantly increased (p < 0.05 vs control group) and AM significantly decreased (p < 0.05 vs. IR group) all of the biochemical parameters. Immunohistological evaluation showed that AM attenuated morphological changes as apoptosis associated with kidney injury. The results of this study indicate that AM attenuates both biochemically and immunohistopathologically kidney injury induced by aortic IR in rats

Keywords

adrenomedullin - renal injury - infrarenal abdominal aorta - ischemia-reperfusion
 

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