Influence of piperine on ibuprofen inducedantinociception and its pharmacokinetics

Abstract

Piperine (CAS 94-62-2), an alkaloid obtained from Piper nigrum and P. longum, is a known inhibitor of various enzymes (CYP isozymes) responsible for biotrans- formation of drugs. By inhibiting the metabolism of drugs, piperine improves the bioavailability of drugs. In the present study piperine (10 mg/kg) significantly increased the dose-dependent antinociceptive activity of ibuprofen evaluated by both acetic acid writhing and formalin test, when it was administered with ibuprofen. Ibuprofen plasma concentration was also increased when it was administered with piperine. The synergistic antinociception activity of ibuprofen when administered with piperine can be attributed to increased plasma concentration of ibuprofen. From this study it can be concluded that piperine can be used as a bioenhancer along with ibuprofen.

Key words

Bioenhancer - Ibuprofen - Piperine - Synergistic antinociception

• References

• 1 Singh R, Devi S, Patel JH, Patel UD, Bhavsar SK, Thaker AM. Indian herbal bioenhancers: a review. Pharmacog Rev. 2009; 3: 90-2
• 2 Bano G, Raina RK, Zutshi U, Bedi KL, Johri RK, Sharma SC. Effect of piperine on bioavailability and pharmacokineticsof propranolol and theophylline in healthy volunteers. Eur J Clin Pharmacol. 1991; 41: 615-7
• 3 Khan IA, Mirza ZM, Kumar A, Verma V, Qazi GN. Piperine, a phytochemical potentiator of ciprofloxacin against Staphylococcus aureus. Antimicrob Agents Chemother. 2006; 50: 810-2
• 4 Atal N, Bedi KL. Bioenhancers: revolutionary concept tomarket. J Ayurveda Integrative Med. 2010; 2: 96-99
• 5 Rang HP, Dale MM, Ritter JM, Moore PK In: Pharmacology. 2nd edition Elsevier India; 2005: 546
• 6 Adams SS. The propionic acids: a personal perspective. J Clin Pharmacol. 1992; 32: 317-23
• 7 Siegmund EA, Cadmus RA, Lu G. A method for evaluatingboth non-narcotic and narcotic analgesics. Proc Soc Exp Biol Med. 1957; 95: 729-31
• 8 Koster R, Andersin N, Debber EJ. Acetic acid for analgesicsscreening. Fed Proc. 1959; 18: 412-
• 9 Gupta SK, Velpandian T, Sengupta S, Mathur P, Sapra P. Influence of piperine on nimesulide induced antinocicep-tion. Phytother Res. 1998; 12: 266-9
• 10 Dubuisson D, Dennis SG. The formalin test: a quantitativestudy of the analgesic effects of morphine, meperidine and brain stem stimulation in rats and cats. Pain. 1977; 4: 161-74
• 11 Jourdan D, Ardid D, Bardin L. A new automated method ofpain scoring in formalin test in rats. Pain. 1997; 71: 265-70
• 12 Vasudean M, Ravisankar S, Ravibabu T, Nanjan MJ. Simultaneous estimation of paracetamol, methocarbamol and ibuprofen by reversed phase HPLC method. Indian Drugs. 2000; 37: 386-9