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Arzneimittelforschung 2010; 60(6): 336-339
DOI: 10.1055/s-0031-1296297
Antibiotics · Antimycotics · Antiparasitics · Antiviral Drugs · Chemotherapeutics · Cytostatics
Editio Cantor Verlag Aulendorf (Germany)

Development of an analytical method for cefpirome in plasma by simplified HPLC technique and its applications

Somchai Sriwiriyajan
Department of Pharmacology, Faculty of Science, Prince of Songkla University, Songkhla, Thailand
,
Werawath Mahatthanatrakul
Department of Pharmacology, Faculty of Science, Prince of Songkla University, Songkhla, Thailand
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Publikationsverlauf

Publikationsdatum:
02. Dezember 2011 (online)

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Abstract

The objective of this study was to develop a rapid and simplified, reliable high-performance liquid chromatography (HPLC) method for quantification of cefpirome (CAS 98753-19-6) in plasma. After precipitation of the plasma containing the internal standard, hydrochlorothiazide, with 5 % trichloroacetic acid (TCA), the analysis of the cefpirome level in the plasma samples was carried out using a reverse-phase C18 column with the ultraviolet detector set at a wavelength of 258 nm. The chromatographic separation was accomplished with an isocratic mobile phase consisting of acetonitrile-acetate buffer pH 5. The proposed method was specific and sensitive with a lower limit of quantitation (LLOQ) of 0.5 μg/ml. This HPLC method was validated by examining the precision and accuracy for inter- and intra-day analysis in the concentration range 0.5–64.0 μg/ml. The relative standard deviation in the inter-and intra-day validation was less than 3 %. Analytical recovery was more than 84 %, and cefpirome was found to be stable in human plasma during both the storage and assay procedures. A satisfactory pharmacokinetic study of cefpirome was carried out in rabbits using the devised procedure.

Key words

CAS 98753-19-6 - Cefpirome, pharmacokinetics, quantification in plasma - Cephalosporin antibiotic - HPLC
 

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