A method for the rapid and simultaneous determination of 6,7-dimethylesculetin (CAS
120-08-1) and geniposide (CAS 24512-63-8) in rat plasma has been developed, using
validated high performance liquid chromatography (HPLC) with solid phase extraction
(SPE). The HPLC analysis was performed on a commercially available column (200 mm
× 4.6 mm, 5 µm) with acetonitrile-methanol −0.1% aqueous formic acid as mobile phase
and the UV detection at 343 nm and 238 nm for 6,7-dimethylesculetin and geniposide,
respectively. The calibration curves for 6,7-dimethylesculetin and geniposide were
linear over the range 0.4–25.6 µg/mL and 1.12–71.68 µg/mL, respectively. The lower
limits of quantitation were 0.40 µg/mL and 1.12 µg/mL, and the lower limits of detection
were 0.06 µg/mL and 0.09 µg/mL, respectively. The intra-day and inter-day precision
for 6,7-dimethylesculetin and geniposide were < 5%, whereas the absolute recovery
percentages were > 74%. A successful application of the developed HPLC analysis was
demonstrated for the pharmacokinetic study of a Traditional Chinese Medicine formula
of Yin Chen Hao Tang preparation.
Key words
CAS 120-08-1 - CAS 24512-63-8 - 6,7-Dimethylesculetin, high performance liquid chromatography,
pharmacokinetics, rat - Geniposide, high performance liquid chromatography, pharmacokinetics,
rat - Yin Chen Hao Tang preparation, pharmacokinetics
