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Planta Med 2012; 78(6): 557-564
DOI: 10.1055/s-0031-1298212
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Geniposide, an Iridoid Glucoside Derived from Gardenia jasminoides, Protects against Lipopolysaccharide-Induced Acute Lung Injury in Mice

Yang Xiaofeng1 [*] , Cai Qinren1 [*] , He Jingping1 , Chu Xiao1 , Wei Miaomiao1 , Feng Xiangru1 , Xie Xianxing1 , Huo Meixia1 , Liu Jing1 , Wei Jingyuan3 , Ci Xinxin1 , Li Hongyu1 , Deng Yanhong1 , Jiang Lanxiang2 , Deng Xuming1
  • 1Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China
  • 2Department of Dermatology, Second Hospital of Jilin University, Changchun, People's Republic of China
  • 3Liaoning Province Academy of Analytic Science, Shenyang, People's Republic of China
Further Information

Publication History

received July 4, 2011 revised January 1, 2012

accepted January 10, 2012

Publication Date:
21 February 2012 (online)

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Abstract

Geniposide, a main iridoid glucoside component of gardenia fruit, has been shown to possess anti-inflammatory activity. However, its potential use for acute lung injury (ALI) has not yet been studied. The aim of this study was to evaluate the anti-inflammatory properties of geniposide using a mouse ALI model. ALI was induced by intranasal injection of lipopolysaccharide (LPS). Pretreatment of mice with geniposide (20, 40, or 80 mg/kg) resulted in a marked reduction in inflammatory cells and total protein concentration in the bronchoalveolar lavage fluid (BALF) of mice. Levels of inflammatory mediators, including tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10), were significantly altered after treatment with geniposide. Histological studies using hematoxylin and eosin (H&E) staining demonstrate that geniposide substantially inhibited LPS-induced alveolar wall changes, alveolar haemorrhage, and neutrophil infiltration in lung tissue, with evidence of reduced myeloperoxidase (MPO) activity. In addition, we investigated potential signal transduction mechanisms that could be implicated in geniposide activity. Our results suggest that geniposide may provide protective effects against LPS-induced ALI by mitigating inflammatory responses and that the compound's mechanism of action may involve blocking nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinases (MAPK) signalling pathway activation.

Key words

geniposide - lipopolysaccharide - acute lung injury - cytokine - nuclear factor-kappaB - mitogen-activated protein kinases - Gardenia jasminoides - Rubiaceae

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1 Yang Xiaofeng and Cai Qinren contributed equally to this work.

Prof Jiang Lanxiang

Department of Dermatology
Second Hospital of Jilin University

Changchun

Jilin 130041

People's Republic of China

Phone: +86 4 31 88 79 67 11

Email: jianglanxiang@sohu.com

Prof Dr Deng Xuming

Department of Veterinary Pharmacology
College of Animal Science and Veterinary Medicine
Jilin University

Changchun

Jilin 130062

People's Republic of China

Phone: +86 4 31 87 83 61 61

Fax: +86 4 31 87 83 61 60

Email: xumingdeng@jluhp.edu.cn