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Planta Med 2012; 78(10): 962-967
DOI: 10.1055/s-0031-1298579
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

High Molecular Weight Constituents of Cranberry Interfere with Influenza Virus Neuraminidase Activity In Vitro

Esther Oiknine-Djian   *
1   Department of Biochemistry and Molecular Biology, Chanock Center of Virology, IMRIC, Hadassah-Hebrew University Medical and Dental Schools, Jerusalem, Israel
,
Yael Houri-Haddad   *
2   Department of Prosthodontics, Hadassah-Hebrew University Medical and Dental Schools, Jerusalem, Israel
,
Ervin Itshak Weiss
2   Department of Prosthodontics, Hadassah-Hebrew University Medical and Dental Schools, Jerusalem, Israel
,
Itzhak Ofek
3   Department of Clinical Microbiology and Immunology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel
,
Evguenia Greenbaum
1   Department of Biochemistry and Molecular Biology, Chanock Center of Virology, IMRIC, Hadassah-Hebrew University Medical and Dental Schools, Jerusalem, Israel
,
Kevan Hartshorn
4   Department of Medicine, Boston University School of Medicine, Boston, MA, USA
,
Zichria Zakay-Rones
1   Department of Biochemistry and Molecular Biology, Chanock Center of Virology, IMRIC, Hadassah-Hebrew University Medical and Dental Schools, Jerusalem, Israel
› Author Affiliations
Further Information

Publication History

received 27 December 2011
revised 24 April 2012

accepted 25 April 2012

Publication Date:
15 May 2012 (online)

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Abstract

Cranberry juice contains high molecular weight non-dialyzable material (NDM) which was found to inhibit hemagglutination induced by the influenza virus (IV) as well as to neutralize the cytotoxicity of IV in cell cultures. Because influenza virus surface glycoproteins hemagglutinin (HA) and neuraminidase (NA) are involved in viral replication and in the infectious process, we sought in the present study to examine the effect of NDM on neuraminidases which are the target of most anti-influenza drugs today. NDM inhibited the NA enzymatic activity of influenza A and B strains as well as that of Streptococcus pneumoniae. This finding is of importance considering the emergence of influenza isolates resistant to antiviral drugs, reaching 90 % in some places. The anti-NA activity of NDM, evaluated by the MUNANA method and expressed as the concentration required for 50 % inhibition (IC50), was most potent against N1 (IC50, 192 µg/mL), less active against BN and N2 (IC50, 509 µg/mL and 1128 µg/mL, respectively), and moderately active against Streptococcus pneumoniae NA (IC50, 594 µg/mL). The in vitro findings of the present study suggest that cranberry constituents may have a therapeutic potential against both A and B influenza virus infections and might also interfere with the development of secondary bacterial complications.

Key words

cranberry juice - NDM - Vaccinium macrocarpon Ait - Ericaceae - neuraminidase - influenza virus

* These authors contributed equally to the study.


 

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