Voltammetric Determination of Praziquantel in Tablets and Biological Fluids

Mohammed Rizk; Fathalla Belal; Fawzia Ibrahim; Soad Ahmed; Nahed M. El-Enany

doi:10.1055/s-0031-1300099

Arzneimittelforschung, Inhaltsverzeichnis

Arzneimittelforschung 2001; 51(8): 673-678
DOI: 10.1055/s-0031-1300099

Antibiotics · Antiviral Drugs · Chemotherapeutics · Cytostatics

Editio Cantor Verlag Aulendorf (Germany)

Voltammetric Determination of Praziquantel in Tablets and Biological Fluids

Mohammed Rizk

Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt

,

Fathalla Belal

Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt

,

Fawzia Ibrahim

Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt

,

Soad Ahmed

Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt

,

Nahed M. El-Enany

Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt

› Institutsangaben

Abstract

als PDF herunterladen

Summary

A simple method is described for the determination of praziquantel (CAS 55268-74-1) in its pure form, tablet formulations and biological fluids. The proposed method depends upon the polarographic activity of praziquantel at the dropping mercury electrode (DME) in Britton Robinson buffers, whereby a well-defined catholic wave is produced over the pH range 7–12. The wave was characterized as being irreversible diffusion-controlled with limited adsorption properties. The diffusion current constant (Id) was 0.56 ± 0.004 (n = 11). The current-concentration relationship was found to be rectilinear over the range 8–48, 3.2–38.4 and 0.48-20 μg · ml⁻¹ using direct current (DC_t), differential pulse polarographic (DPP) and alternating current (AC_t) odes, respectively, with minimum detection limit (S/N = 2) of 0.32 μg · ml⁻¹ (1.02 × 10⁻⁶ mol/l and 0.02 μg · ml⁻¹ (6.4 × 10⁻⁸ mol/l) for DPP and AC_t modes respectively. The average percent recovery was favourably compared to a reference method with a satisfactory standard deviation. The proposed method was applied to spiked human urine and plasma. The percentage recoveries were 99.33 ± 0.79 and 98.23 ± 0.53, respectively.

Zusammenfassung

Voltammetrische Bestimmung von Praziquantel in Tabletten und biologischen Flüssigkeiten

Es wird ein einfaches Verfahren zur Bestimmung von Praziquantel (CAS 55268-74-1) in Zubereitungen (Tabletten) und biologischen Flüssigkeiten beschrieben, das auf der polarographischen Aktivität an der tropfenden Quecksilberelektrode (DME) in Britton-Robinson-Puffern unter Ausbildung eines klar definierten kathodischen Signals im pH-Bereich 7 bis 12 beruht. Der konstante Diffusionsstrom (Id) betrug 0,56 ± 0,004 (n = 11) und das Strom-Konzentrations-Verhältnis war rektilinear in den Bereichen 8–48 (DC_t), 3,2-38,4 (DPP) und 0,48-20 μg ml⁻¹ (AC_t). Die unteren Nachweisgrenzen (S/ Ν = 2) lagen bei 0,32 μg ml⁻¹ (1,02 × 10⁻⁶ mol/l) für DPP bzw. 0,02 μg ml⁻¹ (6,4 × 10⁻⁸ mol/l) für AC_t. Die durchschnittliche prozentuale Wiederfindungs-rate bei aufgestocktem Humanurin und -serum betrug 99,33 ± 0,79 bzw. 98,23 ± 0,53 %.

Key words

CAS 55268-74-1 - Praziquantel, determination in tablets and biological fluids

PDF (310 kb)