Reliable and Reproducible Murine Models for Commonly Used Abdominal Plastic Surgical Flaps

Abstract

Animal models have been used for many years in surgical research to develop different surgical techniques, improve understanding of anatomy and physiology and hone surgical skills. The benefit of such models has been particularly important in developing relatively young specialties like plastic surgery and many plastic surgical techniques are designed and studied in animals long before they are used in humans. We describe techniques for raising several reliable and reproducible abdominal flaps in rodents, including transverse rectus abdominis myocutaneous flaps in rats and mice, superficial inferior epigastric artery flaps in rats and perforator flaps in rats. The intention of this paper is to act as a point of reference for any microvascular or plastic surgeon who is planning to perform abdominal plastic surgical flap research or further microvascular skills.

Keywords

TRAM - DIEP - SIEA - free flap - mice and rats

• References

• 1 Reid RR, Said HK, Mogford JE, Mustoe TA. The future of wound healing: pursuing surgical models in transgenic and knockout mice. J Am Coll Surg 2004; 199 (4) 578-585
• 2 Agrawal VK, Copeland KM, Barbachano Y , et al. Microvascular free tissue transfer for gene delivery: in vivo evaluation of different routes of plasmid and adenoviral delivery. Gene Ther 2009; 16 (1) 78-92
• 3 McFarlane RM, DeYoung G, Henry RA. Prevention of necrosis in experimental pedicle flaps with hyperbaric oxygen. Surg Forum 1965; 16: 481-482
• 4 Tai Y, Hasegawa H. A transverse abdominal flap for reconstruction after radical operations for recurrent breast cancer. Plast Reconstr Surg 1974; 53 (1) 52-54
• 5 Hartrampf CR, Scheflan M, Black PW. Breast reconstruction with a transverse abdominal island flap. Plast Reconstr Surg 1982; 69 (2) 216-225
• 6 Hallock GG, Rice DC. Comparison of TRAM and DIEP flap physiology in a rat model. Plast Reconstr Surg 2004; 114 (5) 1179-1184
• 7 Kim EK, Hong JP. The effect of recombinant human erythropoietin on ischemia-reperfusion injury: an experimental study in a rat TRAM flap model. Plast Reconstr Surg 2007; 120 (7) 1774-1781
• 8 Ozkan O, Coskunfirat OK, Ozgentas HE, Yildirim I, Dikici MB. Is it possible to increase the survival of the transverse rectus abdominis musculocutaneous flap following previous abdominoplasty using a delay procedure? An experimental study in the rat. Plast Reconstr Surg 2005; 116 (7) 1945-1952
• 9 Sano K, Hallock GG, Rice DC. The relative importance of the deep and superficial vascular systems for delay of the transverse rectus abdominis musculocutaneous flap as demonstrated in a rat model. Plast Reconstr Surg 2002; 109 (3) 1052-1057, discussion 1058–1059
• 10 Wang H, Li Z, Liu X. Effects of various protocols of ischemic preconditioning on rat tram flaps. Microsurgery 2008; 28 (1) 37-43
• 11 Ely PB, Kobayashi LA, Campos JH, Gomes HC, Juliano Y, Ferreira LM. Nicotine on rat TRAM flap. Acta Cir Bras 2009; 24 (3) 216-220
• 12 Seify H, Bilkay U, Jones G. Improvement of TRAM flap viability using human VEGF-induced angiogenesis: a comparative study of delay techniques. Plast Reconstr Surg 2003; 112 (4) 1032-1039
• 13 Lineaweaver WC, Lei MP, Mustain W, Oswald TM, Cui D, Zhang F. Vascular endothelium growth factor, surgical delay, and skin flap survival. Ann Surg 2004; 239 (6) 866-873, discussion 873–875
• 14 Antonini A, Zacchigna S, Papa G, Novati F, Pascone M, Giacca M. Improved survival of rat ischemic cutaneous and musculocutaneous flaps after VEGF gene transfer. Microsurgery 2007; 27 (5) 439-445
• 15 de Freitas AL, Gomes HC, Lisboa BC, Arias V, Han SW, Ferreira LM. Effect of gene therapy with vascular endothelial growth factor after abdominoplasty on TRAM flap viability in a rat model. Plast Reconstr Surg 2010; 125 (5) 1343-1351
• 16 Seify H, Bulky U, Jones G. Effect of vascular endothelial growth factor-induced angiogenesis on TRAM flap harvesting after abdominoplasty. Plast Reconstr Surg 2003; 111 (3) 1212-1216
• 17 Pradka SP, Ong YS, Zhang Y , et al. Increased signs of acute rejection with ischemic time in a rat musculocutaneous allotransplant model. Transplant Proc 2009; 41 (2) 531-536
• 18 Zhang F, Lineaweaver WC, Kao S , et al. Microvascular transfer of the rectus abdominis muscle and myocutaneous flap in rats. Microsurgery 1993; 14 (6) 420-423
• 19 Ozkan O, Koshima I, Gonda K. A supermicrosurgical flap model in the rat: a free true abdominal perforator flap with a short pedicle. Plast Reconstr Surg 2006; 117 (2) 479-485
• 20 Cooley BC, Daley R. Free flap transplantation in mice. Microsurgery 1998; 18 (5) 320-323
• 21 Brown SH, Banuelos K, Ward SR, Lieber RL. Architectural and morphological assessment of rat abdominal wall muscles: comparison for use as a human model. J Anat 2010; 217 (3) 196-202
• 22 Taylor GI, Minabe T. The angiosomes of the mammals and other vertebrates. Plast Reconstr Surg 1992; 89 (2) 181-215
• 23 Hallock GG. The rat TRAM flap: a human analogue?. Plast Reconstr Surg 1995; 96 (1) 233-234