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Pharmacopsychiatry 2013; 46(01): 10-15
DOI: 10.1055/s-0032-1311607
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Ziprasidone – Not Haloperidol – Induces more de-novo Neurogenesis of Adult Neural Stem Cells Derived from Murine Hippocampus

J. Benninghoff
1   Department of Psychiatry, LMU-University of Munich, Munich, Germany
4   Department of Psychiatry, University Hospital of Essen, Essen, Germany
,
H. Grunze
2   Institute of Neuroscience, Newcastle University, Newcastle, United Kingdom
,
C. Schindler
1   Department of Psychiatry, LMU-University of Munich, Munich, Germany
,
J. Genius
1   Department of Psychiatry, LMU-University of Munich, Munich, Germany
,
R. J. Schloesser
3   Laboratory of Molecular Pathophysiology, Mood and Anxiety Disorders Programm, National Institute of Mental Health, NIH, Bethesda, USA
,
A. van der Ven
1   Department of Psychiatry, LMU-University of Munich, Munich, Germany
,
S. Dehning
1   Department of Psychiatry, LMU-University of Munich, Munich, Germany
,
J. Wiltfang
4   Department of Psychiatry, University Hospital of Essen, Essen, Germany
,
H.-J. Möller
1   Department of Psychiatry, LMU-University of Munich, Munich, Germany
,
D. Rujescu
1   Department of Psychiatry, LMU-University of Munich, Munich, Germany
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 22. Dezember 2011
revised 25. Januar 2012

accepted 27. März 2012

Publikationsdatum:
16. Mai 2012 (online)

Auch verfügbar auf
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Abstract

Introduction:

Here, we present a stem-cell based study on the de-novo generation of beta-III-tubulin-positive neurons after treatment with the classic antipsychotic drug haloperidol or after treatment with the second-generation antipsychotic (SGA) ziprasidone.

Methods:

Adult neural stem cells (ANSC) dissociated from the adult mouse hippocampus were expanded in cell culture with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). ANSC differentiated upon withdrawal of EGF and bFGF.

Results and Discussion:

Ziprasidone generated significantly more beta-III-tubulin-positive neurons than haloperidol during the differentiation of adult neural stem cells isolated from murine hippocampus (ANSC). We assume that this net increase in neurogenesis by ziprasidone relies on this drug’s 5-HT1A receptor affinity, which is not present in the haloperidol molecule, since the inactivation by WAY100621 impeded this process. These data could possibly suggest a clinical relevance for studying antipsychotic drugs in the stem cell paradigm employed in this study.

Key words

ziprasidone - haloperidol - beta-III-tubulin-positive neurons - adult neural stem cells - murine hippocampus - de-novo generation
 

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