Planta Med 2012; 78(14): 1543-1548
DOI: 10.1055/s-0032-1315026
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Antinociceptive Effects of a Chloroform Extract and the Alkaloid Dicentrine Isolated from Fruits of Ocotea puberula

Deise Prehs Montrucchio
1   Departamento de Farmácia, Setor de Ciências da Saúde, Universidade Federal do Paraná, Curitiba, PR, Brazil
2   Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
,
Obdulio Gomes Miguel
1   Departamento de Farmácia, Setor de Ciências da Saúde, Universidade Federal do Paraná, Curitiba, PR, Brazil
,
Sandra Maria Warumby Zanin
1   Departamento de Farmácia, Setor de Ciências da Saúde, Universidade Federal do Paraná, Curitiba, PR, Brazil
,
Gabriel Araujo da Silva
3   Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil
,
Alcíbia Maia Cardozo
4   Departamento de Patologia, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil
,
Adair Roberto Soares Santos
2   Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
5   Laboratório de Neurobiologia da Dor e Inflamação, Departamento de Ciências Fisiológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil
› Author Affiliations
Further Information

Publication History

received 07 May 2012
revised 05 June 2012

accepted 08 June 2012

Publication Date:
16 July 2012 (online)

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Abstract

The present work describes the chemical characterization of a chloroform fraction (CF) obtained from an extract of Ocotea puberula (Lauraceae) fruits, and preliminary antinociceptive analysis of CF and the alkaloid dicentrine, isolated from this fraction. CF (30–300 mg/kg, p. o.) caused dose-related inhibition of abdominal constrictions caused by acetic acid and also inhibited both phases of formalin-induced nociception. However, hexane or ethyl acetate fractions did not produce any effect. Antinociception caused by CF (100 mg/kg, p. o.) in the acetic acid test was not affected either by caffeine, an adenosine receptor antagonist, or by naloxone, an opioid receptor antagonist, and neither was associated with nonspecific effects such as muscle relaxation or sedation. Furthermore, dicentrine (30–300 mg/kg, p. o.) produced dose-related inhibition of acetic acid-induced pain without causing changes in the motor performance of mice. The results show, for the first time, that CF from Ocotea puberula fruits produced marked antinociception in different models of chemical pain, and this effect appears to be, at least in part, due to the presence of dicentrine. The mechanism by which CF and the alkaloid produced antinociception still remains unclear, but the adenosinergic or opioid system seems unlikely to be involved in this action.

Supporting Information