Planta Med 2012; 78 - PD116
DOI: 10.1055/s-0032-1320474

Evaluation of antinociceptive activity of Pereskia bleo Kunth

CC Guilhon 1, IRA Wahab 2, 3, F Boylan 2, PD Fernandes 1
  • 1Federal University of Rio de Janeiro, ICB, Brazil
  • 2School of Pharmacy and Pharmaceutical Sciences, Trinity College, Dublin, Ireland
  • 3Faculty of Agro-Industry and Natural Resources, University Malaysia Kelantan, Kelantan, Malaysia

Our aim was to identify the antinociceptive effect for P. bleo leaves as they are popularly used in Malaysia for the treatment of rheumatism, among other conditions. Plant material was collected in Malaysia (2009). An ethanol extract (E) was prepared from leaves (1.4g) and submitted to liquid liquid partition with: hexane [H], ethyl acetate [EA], and butanol [B]. Antinociceptive activity was evaluated by the hot plate test (calculated as area under the curve, AUC) at the doses 30, 50, and 100mg/kg. The mechanism of action was evaluated pre-treating the animals with naloxone (1mg/kg, N). The protocol for the use of animals received the number ICBDFBC015. Statistical analyses were performed by ANOVA and Bonferroni's test (* or # p<0.05 in relation to vehicle or fraction-treated group, respectively). Results:

mg/kg

Vehicle

Morphine

E

H

EA

B

30

1,425±159

5,153±581*

1,071±113*

2,268±233*

1,340±141

4,341±450*

50

1,425±159

5,153±581*

2,495±244*

3,306±344*

4,293±401*

3,318±365*

100

1,425±159

5,153±581*

2,826±314*

2,415±256*

3,153±336*

7,292±741*

100 + N

1,219±113

133±42#

1,808±231#

256.5±301#

172.5±55#

1,146±129#

Conclusions: Fractions from P. bleo demonstrated central antinociceptive effect mediated, at least in part, by opioid system. Financial support: CNPq, FAPERJ, and Instituto Vital Brazil.