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DOI: 10.1055/s-0032-1321058
Cytotoxic and antimicrobial activity of Cynoglossum columnae Ten. in vitro roots
Cynoglossum columnae Ten. (Boraginaceae) in vitro root cultures were established as producing naphthoquinone metabolites and investigated for cytotoxic and antimicrobial activity. The crude extracts of postcultured media showed cytotoxic activity against three cancer cell lines (HeLa, HL-60, HCT-116). The highest antimicrobial activity was observed against Staphylococcus spp., Stenotrophomonas maltophilia and Candida spp. strains. Two secondary metabolites of naphthoquinone type, present in the majority of tissues and postculture media, have been isolated and biologically investigated under the same conditions. The comparison of results showed 5O,6-(isohex-1-en-1,2-diyl)-2-methoxynaphthazarin as the most potent in cytotoxic activity towards all three investigated cancer cell lines (IC50 –2,0µg/ml for HL-60). The structure elucidation of second isolated naphthoquinone derivative is in progress.