Subscribe to RSS
DOI: 10.1055/s-0032-1327879
Antibiofilm Activity of Dendrophthoe falcata against Different Bacterial Pathogens
Publication History
received 31 January 2012
revised 26 August 2012
accepted 26 September 2012
Publication Date:
31 October 2012 (online)
![](https://www.thieme-connect.de/media/plantamedica/201218/lookinside/thumbnails/10.1055-s-0032-1327879-1.jpg)
Abstract
Dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. In this context, the validations of the traditional claim that the leaf extract of D. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. The bacterial biofilms were quantified by crystal violet staining. Among the 17 bacterial pathogens screened, the methanolic fraction of the leaf extract clearly demonstrated antibiofilm activity for Proteus mirabilis, Vibrio vulnificus, Aeromonas hydrophila, Shigella sonnei, Chromobacterium violaceum ATCC 12472, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Vibrio cholerae, and Proteus vulgaris. At biofilm inhibitory concentrations, biofilm formation was reduced by up to 70–90 %. Furthermore, the potential quorum-sensing activity of the leaf extract was tested by agar well diffusion using Chromobacterium violaceum (ATCC 12472 & CV O26) reporter strains. The inhibition of violacein production may be due to direct or indirect interference on QS by active constituents or the interactive effect of different phytocompounds present in the extracts. This is the first report on antibiofilm and QS activity of D. falcata leaf extracts, signifying the scope for development of complementary medicine for biofilm-associated infections.
-
References
- 1 Tart AH, Wozniak DJ. Shifting paradigms in Pseudomonas aeruginosa biofilm research. In: Romeo T, editor Bacterial biofilms. Current topics in microbiology and immunology. Berlin, Heidelberg: Springer-Verlag; 2008: 193-206
- 2 Bjarnsholt T, Nielsen TT, Høiby N, Givskov M. Interference of Pseudomonas aeruginosa signalling and biofilm formation for infection control. Exp Rev Mol Med 2010; 12: e11
- 3 Gawande PV, LoVetri K, Yakandawala N, Romeo T, Zhanel GG, Cvitkovitch DG, Madhyastha S. Antibiofilm activity of sodium bicarbonate, sodium metaperiodate and SDS combination against dental unit waterline-associated bacteria and yeast. J Appl Microbiol 2008; 105: 986-992
- 4 Rasooli I, Fakoor MH, Yadegarinia D, Gachkar L, Allameh A, Rezaei MB. Antimycotoxigenic characteristics of Rosmarinus officinalis and Trachyspermum copticum L. essential oils. Int J Food Microbiol 2008; 122: 135-139
- 5 Limsuwan S, Voravuthikunchai SP. Boesenbergia pandurata (Roxb.) Schltr., Eleutherine americana Merr. and Rhodomyrtus tomentosa (Aiton) Hassk. as antibiofilm producing and antiquorum sensing in Streptococcus pyogenes . FEMS Immunol Med Microbiol 2008; 5: 429-436
- 6 Hasan H, Bahram D. Evaluation of antibiofilm activity of Dentol. Acta Med Iranica 2009; 47: 35-40
- 7 Guarrera PM. Traditional phytotherapy in central Italy (Marche, Abruzzo, and Latium). Fitoterapia 2005; 76: 1-25
- 8 Agarwal DP. Himalayan medicine system and its material medica. Delhi: Indian Publishers and Distributors; 2001: 15
- 9 Pattanayak SP, Sunita P. Wound healing, anti-microbial and antioxidant potential of Dendropthoe falcata (L.f) Ettingsh. J Ethnopharmacol 2008; 120: 241-247
- 10 Mallavadhani UV, Narashimhan K, Mohapatra A, Breeman RBV. New pentacyclic triterpenes and some flavanoids from the fruits of Indian ayurvedic plant Dendrophthoe falcata and their receptor binding activity. Chem Pharm Bull 2006; 54: 740-744
- 11 Bhattarai NK. Folk herbal medicines of Makawanpur district, Nepal. Int J Pharm 1991; 29: 284-295
- 12 You J, Xue X, Cao L, Lu X, Wang J, Zhang L, Zhou SV. Inhibition of Vibrio biofilm formation by a marine actinomycete strain A66. Appl Microbiol Biotechnol 2007; 76: 1137-1144
- 13 Ahmad I, Zahin M, Aqil F, Khan MSA. Novel approaches to combat drug resistant bacteria. In: Ahmad I, Aqil F, editors New strategies combating bacterial infections. New York: Wiley-Blackwell; 2009: 47
- 14 Nostro A. Activity of plant extracts and plant-derived compounds against drug-resistant microorganisms. In: Ahmad I, Aqil F, Owais M, editors Modern phytomedicine. Weinheim: Wiley-VCH; 2006: 199
- 15 Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun 1982; 37: 318-326
- 16 Prashant GM, Chandu GN, Murulikrishna KS, Shafiulla MD. The effect of mango and neem extract on four organisms causing dental caries: Streptococcus mutans, Streptococcus salivarius, Streptococcus mitis and Streptococcus sanguis: an in vitro study. Indian J Dent Res 2007; 18: 148-151
- 17 Alcaraz LE, Satorres SE, Lucero RM, Centorbi ONP. Species identification, slime production and oxacillin susceptibility in coagulase-negative staphylococci isolated from nosocomial specimens. Braz J Microbiol 2003; 34: 45-51
- 18 Lembke C, Podbielski A, Hidalgo-Grass C, Jonas L, Hanski E, Kreikemeyer B. Characterization of biofilm formation by clinically relevant serotypes of group A Streptococci. Appl Environ Microbiol 2006; 72: 2864-2875
- 19 Choo JH, Rukayadi Y, Hwang JK. Inhibition of bacterial quorum sensing by vanilla extract. Lett Appl Microbiol 2006; 42: 637-641
- 20 Nair R, Chanda SV. Antibacterial activity of some medicinal plants of Sourastra region. J Tiss Res 2004; 4: 117-120
- 21 Pandit K, Langfield RD. Antibacterial activity of some Italian medicinal plant. J Ethnopharmacol 2004; 82: 135-142
- 22 Siwakoti M, Varma SK. Plant diversity of eastern Nepal: flora of plains of eastern Nepal. India: M/S Bishen Singh Mahendra Pal Singh; 1999: 491
- 23 Van Delden C, Iglewski BH. Cell-to-cell signalling and Pseudomonas aeruginosa infections. Emerg Infect Dis 1998; 4: 551-560
- 24 Ramachandran NAG, Krishnakumary P. Flavonoids of Dendrophthoe falcata Ettingsh growing on different host plants. Indian J Chem 1989; 29: 584-585
- 25 Simões M, Simões LC, Vieira MJ. A review of current and emergent biofilm control strategies. Food Sci Technol 2010; 43: 573-583
- 26 Zhu J, MeKalanos JJ. Quorum sensing-dependent biofilms enhance colonization in Vibrio cholerae . Dev Cell 2003; 5: 647-656
- 27 Austin B, Zhang XH. Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Lett Appl Microbiol 2006; 43: 119-124
- 28 Brooun A, Liu S, Lewis K. A dose-response study of antibiotic resistance in Pseudomonas aeruginosa biofilms. Antimicrob Agents Chemother 2000; 44: 640-646
- 29 Balunas MJ, Kinghorn AD. Drug discovery from medicinal plants. Life Sci 2005; 78: 431-441
- 30 Saxena HO, Brahmam M. The flora of Orissa. India: Capital Business Services Consultancy; 1995: 1578-1580
- 31 Indrani N, Balasubhramanian K. Chemical examination of Dendropthoe falcata growing on Terminalia tomentosa . Leather Sci 1985; 32: 206
- 32 Indrani N, Rao VSS, Balasubramanian K, Reddy KK, Ramayya TV. The systemic identification of flavanoids. Leather Sci 1980; 27: 438
- 33 Manandhar NP. A contribution to the ethnobotany of Mushar tribes of Dhanusa district, Nepal. J Nat Hist Mus 1986; 10: 53-64
- 34 Boonsong C, Wright SE. The cardiac glycosides present in mistletoe growing on Nerium oleander . Aust J Chem 1961; 14: 449-451
- 35 Anjaneyula ASR, Row LR, Reddy DS. Chemical constitutes of Loranthus falcatus L.f. Curr Sci 1977; 24: 850-851
- 36 Vattem DA, Mihalik K, Crixell SH, McClean RJC. Dietary phytochemicals as quorumsensing inhibitors. Fitoterapia 2007; 78: 302-310
- 37 Toyota M, Asakawa Y. Sesquiterpenoids and cyclic bis(bibenzyls) from the Pakistani liverwort Plagiochasma appendiculatum . J Hattori Bot Lab 1999; 86: 161-167
- 38 Singh BN, Singh BR, Singh RL, Prakash D, Dhakarey R, Upadhyay G, Singh HB. Oxidative DNA damage protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera . Food Chem Toxicol 2009; 47: 1109-1116