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Planta Med 2013; 79(02): 175-180
DOI: 10.1055/s-0032-1328079
Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Antibodies against Yarivʼs Reagent for Immunolocalization of Arabinogalactan-Proteins in Aerial Parts of Echinacea purpurea

Esther Marie Goellner
1   Pharmaceutical Institute, Department of Pharmaceutical Biology, Christian-Albrechts-University of Kiel, Kiel, Germany
,
Jean Christian Gramann
1   Pharmaceutical Institute, Department of Pharmaceutical Biology, Christian-Albrechts-University of Kiel, Kiel, Germany
,
Birgit Classen
1   Pharmaceutical Institute, Department of Pharmaceutical Biology, Christian-Albrechts-University of Kiel, Kiel, Germany
› Author Affiliations
Further Information

Publication History

received 30 August 2012
revised 06 November 2012

accepted 28 November 2012

Publication Date:
08 January 2013 (online)

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Abstract

Arabinogalactan-proteins are glycoproteins that occur in higher plants and are involved in important processes like cell differentiation and plant growth. In the medicinal plant Echinacea purpurea L., they belong to the putative immunomodulating compounds and are structurally well characterized. For microscopic localization of arabinogalactan-proteins, synthetic (β-D-Glc)3 Yariv phenylglycoside that specifically binds to most plant arabinogalactan-proteins was used to label arabinogalactan-proteins in fresh cut sections of stems and petioles of Echinacea purpurea. Polyclonal antibodies against (β-D-Glc)3 Yariv phenylglycoside were used to detect the arabinogalactan-protein-(β-D-Glc)3 Yariv phenylglycoside complex. After addition of fluorescein isothiocyanate-conjugated secondary antibodies, the sections were analyzed by confocal laser scanning microscopy. Arabinogalactan-proteins are localized mainly in the central cylinder in the collateral vascular bundles, especially in the area of the xylem. In cell walls of fully differentiated vessels and tracheids, arabinogalactan-proteins have been detected mainly at the inner area of the wall close to the cell lumina. Intense labeling occurs around pit canals connecting adjacent vessels. Furthermore, arabinogalactan-proteins are present in the lumina of cells of the sclerenchyma caps and in companion cells of the phloem.

Key words

Echinacea purpurea - Asteraceae - immunolocalization - arabinogalactan-proteins - β-D-glucosyl Yariv reagent
 

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