Abstract
Gentiana triflora is widely used to treat inflammation, jaundice,
hepatitis, and rheumatism. In this study, three new compounds, including a
benzo seven-membered ring compound, gentioxepine (1), two secoiridoid
glucosides, (1S,5R,9R)-deglucosyltrifloroside (2) and
(1S,5R,9R)-scabraside (3), together with seven known ones,
(+)-syringaresinol (4), deglucogelidoside (5),
3,4-dihydro-1H,6H,8H-naphtho[1,2-c:4,5-c',d']dipyrano-1,8-dione (6),
deglucoscabraside (7), 2-hydroxy-3-O-β-D-glucosyloxy benzoic
acid methyl ester (8), gentiolactone (9), and trifloroside
(10), were isolated from the ethanol extract of Gentiana
triflora. Their structures were mainly confirmed on the basis of
NMR, MS, IR, CD, and UV spectral evidences. Inhibiting activities of nitric
oxide production of eight of the compounds isolated, as well as
gentiopicroside, were evaluated in the macrophage cell line RAW 264.7. The
results show that the three new compounds and compound 7 could
significantly suppress lipopolysaccharide-induced production of NO, with
IC50s of 2.2 µM, 37.5 µM, 17.6 µM and 6.9 µM, respectively.
Among them, compounds 1, 3, and 7 showed stronger inhibitory
activity than that of the clinically used drug indometacin. Other tested
compounds exerted moderate inhibiting activities.
Key words
Gentiana triflora (Gentianaceae) - secoiridoid glucoside - nitric oxide production - RAW 264.7 macrophage cells
