Planta Med 2013; 79 - PE1
DOI: 10.1055/s-0033-1348569

Abietane Diterpene Isolated from Hyptis Carvalhoi Harley (Lamiaceae) Induces Cell Death by Autophagy and Apoptosis

A Jérsia Araújo 1, JDB Marinho Filho 1, KSB de Lima 2, ER Silveira 2, M Odorico de Moraes 1, C Pessoa 1, LV Costa-Lotufo 1
  • 1Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, CE, Brazil
  • 2Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza, CE, Brazil

HCRH is an abietane diterpene quinone isolated from the roots of Hyptis carvalhoi Harley, an endemic plant to Bahia state (Brazil) to which no phytochemical investigation has so far been reported. In the present work, we evaluated HCRH activity in human adenocarcinoma HCT-116 cell line. HCRH showed cytotoxic effect in a time-dependent manner with IC50 values ranging from 18.5 to 4µM after 24 and 72h of incubation, respectively. HCRH also arrested cells in G0/G1 phase of the cell cycle and induced cell death by autophagy and apoptosis without causing direct damage to DNA. Further studies are in progress to determine its mechanism of action. Financial Support: CNPq, CAPES, PRONEX.

Figure 1: (A) Molecular structure of HCRH; (B) IC50 values after 24h, 48h and 72h of incubation with HCRH for HCT-116 cell lines; (C) Microscopic analysis of differential-stained HCT-116 cells treated with HCRH (6µM) after 48h.