Effects of Local Vibration on Bone Loss in ­Tail-Suspended Rats

L.-w. Sun; H.-q. Luan; Y.-f. Huang; Y. Wang; Y.-b. Fan

doi:10.1055/s-0033-1358468

International Journal of Sports Medicine, Table of Contents

Int J Sports Med 2014; 35(07): 615-624
DOI: 10.1055/s-0033-1358468

Orthopedics & Biomechanics

Effects of Local Vibration on Bone Loss in Tail-Suspended Rats

L.-w. Sun

¹Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, China

,

H.-q. Luan

¹Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, China

,

Y.-f. Huang

¹Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, China

,

Y. Wang

¹Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, China

,

Y.-b. Fan

¹Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, China

› Author Affiliations

Abstract

We investigated the effects of vibration (35 Hz, 45 Hz and 55 Hz) as countermeasure locally applied to unloading hind limbs on bone, muscle and Achilles tendon. 40 female Sprague Dawley rats were divided into 5 groups (n=8, each): tail-suspension (TS), TS plus 35 Hz/0.3 g vibration (TSV35), TS plus 45 Hz/0.3 g vibration (TSV45), TS plus 55 Hz/0.3 g vibration (TSV55) and control (CON). After 21 days, bone mineral density (BMD) and the microstructure of the femur and tibia were evaluated by μCT in vivo. The biomechanical properties of the femur and Achilles tendon were determined by a materials testing system. Ash weight of bone, isotonic contraction and wet weight of soleus were also investigated. 35 Hz and 45 Hz localized vibration were able to significantly ameliorate the decrease in trabecular BMD (expressed as the percentage change from TS, TSV35: 48.11%, TSV45: 31.09%), microstructure and ash weight of the femur and tibia induced by TS. Meanwhile, 35 Hz vibration significantly improved the biomechanical properties of the femur (57.24% bending rigidity and 41.66% Young’s modulus vs. TS) and Achilles tendon (45.46% maximum load and 66.67% Young’s modulus vs. TS). Additionally, Young’s modulus of the femur was highly correlated with microstructural parameters. Localized vibration was useful for counteracting microgravity-induced musculoskeletal loss. In general, the efficacy of 35 Hz was better than 45 Hz or 55 Hz in tail-suspended rats.

Key words

local vibration - bone - microstructure - tendon - muscle

Full Text

References

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Effects of Local Vibration on Bone Loss in ­Tail-Suspended Rats

Effects of Local Vibration on Bone Loss in Tail-Suspended Rats