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Drug Res (Stuttg) 2014; 64(08): 406-411
DOI: 10.1055/s-0033-1358758
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Comparative Analysis of Serum Malondialdehyde, Antioxidant Vitamins and Immunoglobulin Levels in Patients Suffering from Generalized Anxiety Disorder

M. R. Islam
1   Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh
,
M. U. Ahmed
1   Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh
,
M. S. Islam
2   Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali, Bangladesh
,
M. S. B. Sayeed
1   Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh
,
F. Sadia
3   Institute of Statistical Research and Training, University of Dhaka, Dhaka, Bangladesh
,
Z. S. Chowdhury
1   Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh
,
Z. Nahar
4   Department of Pharmacy, Northern University Bangladesh, Dhaka, Bangladesh
,
A. Hasnat
1   Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 12. September 2013

accepted 29. Oktober 2013

Publikationsdatum:
27. November 2013 (online)

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Abstract

The relationship between the elevated levels of serum malondialdehyde, depleted level of antioxidants (vitamin A, E and C) and altered level of immunoglobulins (IgA, IgG and IgM) in several psychiatric disorders has been established by various experimental evidences over the past few years. But previously no study was carried out to determine these components in patients suffering from generalized anxiety disorder (GAD) in Bangladesh. This study was conducted to compare the serum concentration of these components in GAD patients and healthy volunteers; matched by socioeconomic and sociodemographic parameters. Serum level of malondialdehyde and vitamin C were determined by UV spectrophotometric method, vitamins A and E were detected by RP-HPLC method whereas immunoglobulin levels were determined by turbidimetric method. Data were analyzed by independent t-test, Pearson’s correlation and regression analysis. Significantly lower level of vitamin E (p<0.05) and significantly higher level of vitamin C were found in GAD patients than the healthy controls, whereas the change of vitamin A was insignificant. Serum malondialdehyde content was significantly higher (p<0.05) and IgM level was significantly (p<0.05) higher than that of the controls. Change in concentrations of IgG and IgA were insignificant (p>0.05). Pearson’s correlation coefficient suggested that there were some significant positive and negative correlations among these tested components. Our study reveals that GAD patients have considerably higher level of malondialdehyde, immunoglobulins and altered level of antioxidant vitamins. These findings may play a key role in the diagnosis and treatment of GAD patients.

Key words

malondialdehyde - vitamin A - vitamin E - vitamin C - immunoglobulins - generalized anxiety disorder
 

  • References

  • 1 Torpy JM, Burke AE, Golub RM. Generalized Anxiety Disorder. JAMA 2011; 305: 522-522
    CrossrefPubMedGoogle Scholar
  • 2 Firoze AHM, Karim ME, Alam MF et al. Prevalence, Medical care, awareness and attitude towards mental illness in Bangladesh. Bangladesh Journal of psychiatry 2006; 20: 9-31
    PubMedGoogle Scholar
  • 3 Wittchen HU, Zhao S, Kessler RC. DSM-III-R generalized anxiety disorder in the National Comorbidity Survey. Arch Gen Psychiatry 1994; 51: 355-364
    CrossrefPubMedGoogle Scholar
  • 4 Massion AO, Warshaw MG, Keller MB. Quality of life and psychiatric morbidity in panic disorder and generalized anxiety disorder. American Journal of Psychiatry 1993; 150: 600-607
    CrossrefPubMedGoogle Scholar
  • 5 Bouayed J, Rammal H, Soulimani R. Oxidative stress and anxiety Relationship and cellular pathways. Oxid Med Cell Longev 2009; 2: 63-67
    CrossrefPubMedGoogle Scholar
  • 6 Bilici M, Efe H, Koroglu MA et al. Antioxidative enzyme activities and lipid peroxidation in major depression: Alterations by antidepressant treatments. Journal of Affective Disorders 2001; 64: 43-51
    CrossrefPubMedGoogle Scholar
  • 7 Ersan S, Bakir S, Ersan E et al. Examination of free radical metabolism and antioxidant defense system elements in patients with OCD. Progress in Neuro-Psychopharmacology and Biological Psychiatry 2006; 30: 1039-1042
    CrossrefPubMedGoogle Scholar
  • 8 Mahadik SP, Mukherjee S. Free radical pathology and antioxidant defence in schizophrenia: A review. Schizophrenia Research 1996; 19: 1-17
    CrossrefPubMedGoogle Scholar
  • 9 Shohag HM, Ullah MA, Azad MA et al. Serum Antioxidant Vitamins and Malondialdehyde Levels in Patients with Obsessive-Compulsive Disorder. German Journal of Psychiatry 2012; 15: 10-14
    PubMedGoogle Scholar
  • 10 Saito A, Maier CM, Narasimhan P et al. Oxidative stress and neuronal Death/survival signaling in cerebral ischemia. Molecular Neurobiology 2005; 31: 105-116
    CrossrefPubMedGoogle Scholar
  • 11 Ozcankaya R, Delibas N. Malondialdehyde, superoxide dismutase, melatonin, iron, copper, and zinc blood concentrations in patients with Alzheimer disease: cross-sectional study. Croatian Medical Journal 2002; 43: 28-32
    PubMedGoogle Scholar
  • 12 Aksenov MY, Aksenova MV, Butterfield DA et al. Protein oxidation in the brain in Alzheimer’s disease. Neuroscience 2001; 103: 373-383
    CrossrefPubMedGoogle Scholar
  • 13 Dadheech G, Mishra S, Gautam S et al. Oxidative stress, α-tocopherol, ascorbic acid and reduced glutathione status in schizophrenics. Indian Journal of Clinical Biochemistry 2006; 21: 34-38
    PubMedGoogle Scholar
  • 14 Dakhale GN, Khanzode SD, Khanzode SS et al. Supplementation of vitamin C with atypical antipsychotics reduces oxidative stress and improves the outcome of schizophrenia. Psychopharmacology 2005; 182: 494-498
    CrossrefPubMedGoogle Scholar
  • 15 Sivrioglu EY, Kirli S, Sipahioglu D et al. The impact of omega-3 fatty acids, vitamins E and C supplementation on treatment outcome and side effects in schizophrenia patients treated with haloperidol: an open-label pilot study. Progress in Neuropsychopharmacology and Biological Psychiatry 2007; 31: 1493-1499
    CrossrefPubMedGoogle Scholar
  • 16 Maes M, De-Vos N, Pioli R et al. Lower serum Vitamin E concentrations in major depression: Another marker of lowered antioxidant defenses in that illness. Journal of Affective Disorder 2000; 58: 241-246
    CrossrefPubMedGoogle Scholar
  • 17 Miljevic CD, Nikolic A, Damjanovlc A et al. Vitamin C and vitamin E In schizophrenic patients. Biological Psychiatry 1997; 42: 96S
    PubMedGoogle Scholar
  • 18 Altuntas I, Aksoy H, Coskun I et al. Erythrocyte Superoxide Dismutase and Glutathione Peroxidase Activities, and Malondialdehyde and Reduced Glutathione Levels in Schizophrenic Patients. Clinical Chemistry and Laboratory Medicine 2000; 38: 1277-1281
    PubMedGoogle Scholar
  • 19 Atmaca M, Kuloglu M, Tezcan E et al. Antioxidant enzyme and malondialdehyde levels in patients with social phobia. Psychiatry Research 2008; 159: 95-100
    CrossrefPubMedGoogle Scholar
  • 20 Hossain I, Sadat N, Hossain K et al. Serum immunoglobulin profile in somatization disorder patients. German Journal of Psychiatry 2007; 10: 13-17
    PubMedGoogle Scholar
  • 21 Bergquist MS, Rankin-Hill L, Sanchez-Ayendez M et al. Across cultural comparison of adaptation to chronic pain among Anglo-American and native PuertoRican. Med Anthropol 1995; 16: 141-173
    PubMedGoogle Scholar
  • 22 Khanam M, Azad MAK, Ullah MA et al. Serum immunoglobulins profiles in conversion disorder patients. German journal of psychiatry 2008; 11: 141-145
    PubMedGoogle Scholar
  • 23 Frasure A, Smith N. Depression and anxiety. Circulation 2000; 101: 1919-1924
    CrossrefPubMedGoogle Scholar
  • 24 Tiwari SC, Lal N, Trivedi JK et al. Relationship of immunoglobulins with the number and duration of schizophrenic episodes. Indian Journal of Medical Research 1989; 90: 229-232
    PubMedGoogle Scholar
  • 25 Taylor K. Immuno-biochemical interactions in schizophrenia. Schizophr Res 2000; 44: 245-246
    CrossrefPubMedGoogle Scholar
  • 26 Delsi LE, Wyatt RJ. Neurochemical aspects of schizophrenia. Handbook of Neurochemistry 1984; 10: 15-19
    PubMedGoogle Scholar
  • 27 Baker A, Sadat AFMN, Hossain MI et al. Immunoglobulin levels in manic patients. Dhaka Univ J Pharm Sci 2005; 4: 1816-1820
    PubMedGoogle Scholar
  • 28 Hossain I, Sadat N, Hossain K et al. Serum immunoglobulin profile in somatization disorder patients. German Journal of Psychiatry 2007; 10: 13-17
    PubMedGoogle Scholar
  • 29 Nahar Z, Azad MAK, Rahman MA et al. Comparative Analysis of Serum Manganese, Zinc, Calcium, Copper and Magnesium Level in Panic Disorder Patients. Biol Trace Elem Res 2010; 133: 243-363
    CrossrefPubMedGoogle Scholar
  • 30 Islam MR, Ahmed MU, Mitu SA et al. Comparative analysis of serum zinc, copper, manganese, iron, calcium, and magnesium level and complexity of interelement relations in generalized anxiety disorder patients. Biol Trace Elem Res 2013; 154: 21-27
    CrossrefPubMedGoogle Scholar
  • 31 Bieri JG, Tolliver TG, Contingent GL. Simultaneous determination of α-tocopherol and retinol in plasma or red cells by HPLC. American Journal of Clinical Nutrition 1979; 32: 2143-2149
    PubMedGoogle Scholar
  • 32 Nazrul Islam SK, Jahangir Hossain K, Ahsan M. Serum vitamin E, C and A status of the drug addicts undergoing detoxification: influence of drug habit, sexual practice and lifestyle factors. Eur J Clin Nutr 2001; 55: 1022-1027
    CrossrefPubMedGoogle Scholar
  • 33 Nahar Z, Khanum S, Harun S et al. Immunoglobulin levels in panic disorder patients. Pak J Pharm Sci 2012; 25: 149-153
    PubMedGoogle Scholar
  • 34 Woodman CL. The genetics of panic disorder and generalized anxiety disorder. Annals of Clinical Psychiatry 1993; 5: 231-239
    CrossrefPubMedGoogle Scholar
  • 35 Bal N, Acar ST, Yazici A et al. Altered levels of malondialdehyde and vitamin E in major depressive disorder and generalized anxiety disorder. The journal of psychiatric and neurological sciences 2012; 25: 206-211
    PubMedGoogle Scholar
  • 36 Atmaca M, Tezcan E, Kuloglu M et al. Antioxidant enzyme and malondialdehyde values in social phobia before and after citalopram treatment. Eur Arch Clin Neurosci 2004; 254: 231-235
    PubMedGoogle Scholar
  • 37 Kuloglu M, Atmaca M, Tezcan E et al. Antioxidant enzyme and malondialdehyde levels in patients with panic disorder. Neuropsychobiology 2002; 46: 186-189
    CrossrefPubMedGoogle Scholar
  • 38 Behl A, Swami G, Sircar SS et al. Correlation between lipid peroxidation-induced TBARS level and disease severity in obsessive-compulsive disorder. Neuropsychobiology 2010; 61: 210-214
    CrossrefPubMedGoogle Scholar
  • 39 Zhang XY, Tan YL, Cao LY et al. Antioxidant enzymes and lipid peroxidation in different forms of schizophrenia treated with typical and atypical antipsychotics. Schizophrenia Research 2006; 81: 291-300
    CrossrefPubMedGoogle Scholar
  • 40 Kartha VN, Krishnamurthy S. Antioxidant function of vitamin A. International Journal for Vitamin and Nutrition Research 1977; 47: 394-401
    PubMedGoogle Scholar
  • 41 Ricciarelli R, Argellati F, Pronzato MA et al. Vitamin E and neurodegenerative diseases. Molecular Aspects of Medicine 2007; 28: 591-606
    CrossrefPubMedGoogle Scholar
  • 42 Hovatta I, Juhila J, Donner J. Oxidative stress in anxiety and comorbid disorders. Neuroscience research 2010; 68: 261-275
    CrossrefPubMedGoogle Scholar
  • 43 Ranjana KS, Negi R, Pande D et al. Markers of Oxidative Stress in Generalized Anxiety Psychiatric Disorder: Therapeutic Implications. Journal of stress physiology & biochemistry 2012; 8: 32-38
    PubMedGoogle Scholar
  • 44 Felicity Ng, Berk M, Dean O et al. Oxidative stress in psychiatric disorders: evidence base and therapeutic implications. The International Journal of Neuropsychopharmacology 2008; 11: 851-876
    PubMedGoogle Scholar
  • 45 Machlin LJ, Bendich A. Free radical tissue-damage: protective role of antioxidant nutrients. FASEB Journal 1987; 1: 441-445
    PubMedGoogle Scholar
  • 46 Gautam M, Agrawal M, Gautam M et al. Role of antioxidants in generalised anxiety disorder and depression. Indian J Psychiatry 2012; 54: 244-247
    CrossrefPubMedGoogle Scholar
  • 47 Sane AS, Chawla MS, Chokshi SA et al. Serum immunoglobulin status of psychiatric in-patients. Panminerva Med 1990; 32: 88-91
    PubMedGoogle Scholar
  • 48 Ramesh C, Yeragani VK, Balan R et al. A comparative study of immune status in panic disorder patients and controls. Aeta Psychiatr Scand 1991; 84: 396-397
    PubMedGoogle Scholar
  • 49 Maddison SE, Stewart CC, Reimer CB. The relationship of race, sex, and age to concentrations of serum immunoglobulins expressed in international units in healthy adults in the USA. Bull World Health Organ 1975; 52: 179-185
    PubMedGoogle Scholar
  • 50 Kaplan HI, Sadock BJ. Comprehensive text book of psychiatry. 6th ed Baltimore: Williams and Wilkins; 1995
    Google Scholar