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Planta Med 2014; 80(04): 277-282
DOI: 10.1055/s-0033-1360400
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Phyllanthus amarus Does Not Affect Hypernociception in Experimental Autoimmune Encephalomyelitis

Graziella Rigueira Molska
1   Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil
,
Giuseppina Negri
1   Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil
,
Lyvia Izaura Gomes Paula-Freire
1   Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil
,
Leandro Pires Araujo
2   Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brazil
,
Daniele Oliveira Köhn
1   Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil
,
Alexandre Salgado Basso
2   Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brazil
,
Elisaldo Araújo Carlini
3   Departamento de Medicina Preventiva, Universidade Federal de São Paulo, São Paulo, Brazil
› Institutsangaben
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Publikationsverlauf

received 30. September 2013
revised 21. Januar 2014

accepted 24. Januar 2014

Publikationsdatum:
07. März 2014 (online)

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Abstract

Multiple sclerosis is an inflammatory disease of the central nervous system. Chronic pain is one of the main symptoms, affecting many patients. Studies show that the lignans or the apolar extracts of Phyllanthus amarus have antinociceptive effects in different animal models. To evaluate the antihypernociceptive effect of a hexanic extract of P. amarus in experimental autoimmune encephalomyelitis in mice, the chemical composition of the hexanic extract was analyzed by gas chromatography mass spectrometry. After EAE induction, animals were treated with the hexanic extract of P. amarus for 26 consecutive days. Motor coordination and mechanical hypernociception were evaluated on alternate days. The principal lignans found were phyllanthin, niranthin, and 5-demethoxyniranthin. The hexanic extract of P. amarus at a dose of 100, 200, or 400 mg/kg did not affect the development of the disease. The motor coordination and pain threshold of the treated animals were not altered in this experiment. In conclusion, in this test, the hexanic extract of P. amarus did not show evidence of antihypernociceptive activity in experimental autoimmune encephalomyelitis.

Key words

Phyllanthus amarus - Euphorbiaceae - pain - hypernociception - experimental autoimmune encephalomyelitis - lignans
 

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