Effect of Vitamin B12 on Functional Recovery and Histopathologic Changes of Tibial Nerve-Crushed Rats

E. Tamaddonfard; A. A. Farshid; F. Samadi; K. Eghdami

doi:10.1055/s-0033-1363219

Drug Research, Inhaltsverzeichnis

Drug Res (Stuttg) 2014; 64(09): 470-475
DOI: 10.1055/s-0033-1363219

Original Article

Effect of Vitamin B₁₂ on Functional Recovery and Histopathologic Changes of Tibial Nerve-Crushed Rats

Autor*innen

E. Tamaddonfard

¹Department of Basic Sciences, Faculty of Veterinary Medicine, Division of Physiology, Urmia University, Urmia, Iran
A. A. Farshid

²Department of Pathobiology, Faculty of Veterinary Medicine, Division of Pathology, Urmia University, Urmia, Iran
F. Samadi

²Department of Pathobiology, Faculty of Veterinary Medicine, Division of Pathology, Urmia University, Urmia, Iran
K. Eghdami

¹Department of Basic Sciences, Faculty of Veterinary Medicine, Division of Physiology, Urmia University, Urmia, Iran

Abstract

Recent studies have suggested a neuroprotective effect for vitamin B₁₂. The present study investigated the effects of vitamin B₁₂, diclofenac and celecoxib in separate and combined treatments on functional recovery of crushed tibial nerve in rats. In ketamine plus xylazin anesthetized rats, right tibial nerve was crushed using a small hemoatatic forceps. Footprints were recorded 1 day before and on days 7, 14 and 21 after induction of nerve injury. Tibial functional index (TFI) was used to evaluate the recovery of tibial nerve function. Histological changes of tibial nerve were investigated by light microscopy. The recovery of TFI values were significantly accelerated with 10 consecutive days treatments with 0.1 and 0.5 mg/kg of vitamin B₁₂, 5 mg/kg of diclofenac and 1 and 5 mg/kg of celecoxib. The severity of Wallerian degeneration was reduced by above-mentioned doses of vitamin B₁₂, diclofenac and celecoxib. Documented effects were observed when 0.1 mg/kg of vitamin B₁₂ was concurrently used with 1 mg/kg of diclofenac and or 0.2 mg/kg of celecoxib. In the present study, vitamin B₁₂, celecoxib and diclofenac (at a high dose) showed neuroprotective effects. Inhibition of cyclooxygenase (COX) 1 and 2 pathways may be involved in neuroprotective effect of vitamin B₁₂.

Key words

vitamin B₁₂ - cyclooxygenase - neuroprotection - peripheral nerve - rats

Volltext

Referenzen

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