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Planta Med 2014; 80(05): 415-418
DOI: 10.1055/s-0034-1368196
Biological and Pharmacological Activity
Letters
Georg Thieme Verlag KG Stuttgart · New York

Polyacetylenes from Radix et Rhizoma Notopterygii Incisi with an Inhibitory Effect on Nitric Oxide Production In Vitro

Martina Blunder
1   Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria
,
Xin Liu
1   Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria
,
Olaf Kunert
2   Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Austria
,
Nora Anna Winkler
1   Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria
,
Andreas Schinkovitz
1   Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria
,
Corinna Schmiderer
3   Institute of Animal Nutrition an Functional Plant Compounds, University of Veterinary Medicine, Vienna, Austria
,
Johannes Novak
3   Institute of Animal Nutrition an Functional Plant Compounds, University of Veterinary Medicine, Vienna, Austria
,
Rudolf Bauer
1   Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 25. März 2012
revised 08. Dezember 2013

accepted 03. Februar 2014

Publikationsdatum:
20. März 2014 (online)

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Abstract

Notopterygium roots (Qiang Huo) have been used in traditional Chinese medicine for treating colds, inflammatory diseases like rheumatoid arthritis, and as an analgesic. The anti-inflammatory activity of the roots of Notopterygium incisum has been evaluated by testing the inhibitory activity on nitric oxide production by inducible nitric oxide synthase. The apparent authenticity of the sample was checked by DNA sequence comparison. Using activity-guided isolation, different compounds were isolated and structurally characterized by means of NMR and mass spectroscopy. Eight polyacetylenes could be identified and were tested on their inhibitory activity on nitric oxide production in RAW 264.7 mouse macrophages using the Griess assay. Different 3-hydroxy allyl polyacetylenes exhibited significant activity (IC50: 8-acetoxyfalcarinol, 20.1 µM; falcarindiol, 9.2 µM; 9-epoxyfalcarindiol, 8.8 µM; and crithmumdiol, 23.6 µM).

Key words

Notopterygium incisum - Apiaceae - polyacetylenes - nitric oxide - iNOS - Griess assay - DNA sequencing

Supporting Information

 

  • References

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