The Effect of Leonurus cardiaca Herb Extract and Some of its Flavonoids on
                    Mitochondrial Oxidative Phosphorylation in the Heart

Jurga Bernatoniene; Dalia M. Kopustinskiene; Valdas Jakstas; Daiva Majiene; Rasa Baniene; Lolita Kuršvietiene; Ruta Masteikova; Arunas Savickas; Adolfas Toleikis; Sonata Trumbeckaite

doi:10.1055/s-0034-1368426

Planta Medica, Table of Contents

Planta Med 2014; 80(07): 525-532
DOI: 10.1055/s-0034-1368426

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

The Effect of Leonurus cardiaca Herb Extract and Some of its Flavonoids on Mitochondrial Oxidative Phosphorylation in the Heart

Jurga Bernatoniene

¹Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania

,

Dalia M. Kopustinskiene

¹Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania

²Laboratory of Biochemistry, Neuroscience Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania

,

Valdas Jakstas

³Department of Pharmacognosy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania

,

Daiva Majiene

¹Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania

²Laboratory of Biochemistry, Neuroscience Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania

,

Rasa Baniene

²Laboratory of Biochemistry, Neuroscience Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania

⁴Department of Biochemistry, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania

,

Lolita Kuršvietiene

⁴Department of Biochemistry, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania

,

Ruta Masteikova

⁵University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Pharmacy, Department of Pharmaceutics, Brno, Czech Republic

,

Arunas Savickas

¹Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania

,

Adolfas Toleikis

²Laboratory of Biochemistry, Neuroscience Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania

,

Sonata Trumbeckaite

²Laboratory of Biochemistry, Neuroscience Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania

³Department of Pharmacognosy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania

› Author Affiliations

Abstract

Motherwort (Leonurus cardiaca) possesses antibacterial, antioxidant, anti-inflammatory, and analgesic activities, and is used as a complementary remedy to improve heart function and blood circulation. Since cardiovascular diseases are often associated with an alteration of mitochondria, the main producers of ATP in cardiac muscle cells, the aim of our work was to determine bioactive constituents present in motherwort aerial parts extract in ethanol and investigate their effects on the functions of cardiac mitochondria. Quantitative determination of polyphenols in L. cardiaca herb extract was performed by HPLC. Mitochondrial respiration rates were evaluated using a Clark-type oxygen electrode. Mitochondrial ROS generation was determined fluorimetrically with Amplex Red and horseradish peroxidase. The results showed that constituents (chlorogenic acid, orientin, quercetin, hyperoside, and rutin) of L. cardiaca herb extract uncouple (by 20–90 %) mitochondrial oxidation from phosphorylation, partially inhibit (by ~ 40 %) the mitochondrial respiratory chain in cases of pyruvate and malate as well as succinate oxidation, and effectively attenuate the generation of free radicals in mitochondria. Since partial uncoupling of mitochondria, respiratory inhibition, and decreased ROS production are proposed as possible mechanisms of cardioprotection, our results imply that L. cardiaca herb extract could be a useful remedy to protect cardiac muscles from the effects of pathogenic processes.

Key words

Leonurus cardiaca - Lamiaceae - flavonoid - polyphenol - oxidative phosphorylation - cardiac mitochondria

Full Text

References

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