Effects of 7-Hydroxycalamenene Isolated from Croton cajucara Essential Oil on
                    Growth, Lipid Content and Ultrastructural Aspects of Rhizopus oryzae

Mariana M. B. Azevedo; Catia A. Almeida; Francisco C. M. Chaves; Galba M. Campos-Takaki; Sonia Rozental; Humberto R. Bizzo; Celuta S. Alviano; Daniela S. Alviano

doi:10.1055/s-0034-1368441

Planta Medica, Inhaltsverzeichnis

Planta Med 2014; 80(07): 550-556
DOI: 10.1055/s-0034-1368441

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Effects of 7-Hydroxycalamenene Isolated from Croton cajucara Essential Oil on Growth, Lipid Content and Ultrastructural Aspects of Rhizopus oryzae

Autor*innen

Mariana M. B. Azevedo^*

¹Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

²Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Catia A. Almeida^*

²Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Francisco C. M. Chaves

³EMBRAPA Amazônia Ocidental, Manaus, AM, Brazil
Galba M. Campos-Takaki

⁴Núcleo de Pesquisas em Ciências Ambientais, Departamento de Química, Universidade Católica de Pernambuco, Recife, PE, Brazil
Sonia Rozental

⁵Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Humberto R. Bizzo

⁶EMBRAPA Agroindústria de Alimentos, Rio de Janeiro, RJ, Brazil
Celuta S. Alviano

²Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Daniela S. Alviano

²Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

Abstract

The leaves and bark of Croton cajucara, a shrub from the Amazon region, have been used in folk medicine to treat diabetes, malaria, and gastrointestinal and liver disorders. The essential oil from the leaves, rich in linalool, presented antileishmanial and antimicrobial activities. A chemotype of this species was found with an essential oil rich in 7-hydroxycalamenene. During our studies of the C. cajucara essential oil, we isolated 7-hydroxycalamenene at > 98 % purity. The minimum inhibitory concentration of 7-hydroxycalamenene against Absidia cylindrospora, Cunninghamella elegans, Mucor circinelloides, Mucor circinelloides f. circinelloides, Mucor mucedo, Mucor plumbeus, Mucor ramosissimus, Rhizopus microsporus, Rhizopus oryzae, and Syncephalastrum racemosum ranged from 19.53 to 2500 µg/mL. The reference drug used, amphotericin B, presented a minimum inhibitory concentration ranging from 0.085 µg/mL to 43.87 µg/mL. 7-Hydroxycalamenene also altered spore differentiation and total lipid content. Ultrastructural analysis by transmission electron microscopy showed significant alterations in the cellular structure of R. oryzae.

Key words

7-Hydroxycalamenene - Rhizopus oryzae - zygomycetes - Croton cajucara - Euphorbiaceae - essential oil

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Referenzen

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