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Drug Res (Stuttg) 2014; 64(12): 680-689
DOI: 10.1055/s-0034-1370965
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Leaf Extract of Markhamia platycalyx: Polyphenolic Profile, Acute Toxicity, Anti-inflammatory, Hepatoprotective and in vitro Antioxidant Activities

R. A. El Dib
1   Department of Pharmacognosy, College of Pharmacy (Girls), King Saud University, Riyadh, Saudi Arabia
2   Department of Pharmacognosy, College of Pharmacy, Helwan University, Cairo, Egypt
,
A. H. Gaara
3   Department of Chemistry of Natural Compounds, National Research Center, Cairo, Egypt
,
S. M. El-Shenawy
4   Department of Pharmacology, National Research Center, Cairo, Egypt
,
J. A. A. Micky
5   Department of Chemistry, Faculty of Science, Alazhar University, Cairo, Egypt
,
A. A. Mohammed
4   Department of Pharmacology, National Research Center, Cairo, Egypt
,
M. S. Marzouk
6   Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
7   Chemistry of Natural Products Group, Center of Excellence for Advanced Sciences National Research Center, Cairo, Egypt
› Author Affiliations
Further Information

Publication History

received 18 January 2014

accepted 13 February 2014

Publication Date:
02 April 2014 (online)

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Abstract

10 polyphenols were identified from 80% aqueous methanol extract (AME) of Markhamia platycalyx [(Baker) Sprague] leaf. Their structures were characterized as protocatechuic acid (1), E-caffeic acid (2), E-methyl caffeate (3), isoverbascoside (4), verbascoside (5), jacraninoside-I (6), cosmosiin (7), cinaroside (8), luteolin (9) and apigenin (10) based on chemical and extensive spectral studies (UV, ESI-MS, 1H, 13C and 1D/2D NMR). Biological evaluation indicated that AME is non-toxic to the experimental animals. It exhibited a significant inhibition of oedema after 1, 2, 3 and 4 h for all examined doses (250, 500 and 1 000 mg/kg). In comparison with silymarin, the AME demonstrated a significant hepatoprotective effect in the form of high reduction in elevated ALT and AST serum levels in regard to paracetamol treated group. As well as, it revealed a marked significant scavenging activity by 62.9, 82.5, 88.3, 83.7 and 83.7%, for the concentrations 20, 40, 60, 80 and 100 mg/ml of the extract, respectively, relative to L-ascorbic acid (86.8%), which was used as a reference antioxidant drug.

Key words

Bignoniaceae - Markhamia platycalyx leaf - acute toxicity - hepatoprotection - anti-inflamatory - in vitro antioxidant
 

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