Leaf Extract of Markhamia platycalyx: Polyphenolic Profile, Acute Toxicity, Anti-inflammatory, Hepatoprotective and in vitro Antioxidant Activities

R. A. El Dib; A. H. Gaara; S. M. El-Shenawy; J. A. A. Micky; A. A. Mohammed; M. S. Marzouk

doi:10.1055/s-0034-1370965

Drug Research, Table of Contents

Drug Res (Stuttg) 2014; 64(12): 680-689
DOI: 10.1055/s-0034-1370965

Original Article

Leaf Extract of Markhamia platycalyx: Polyphenolic Profile, Acute Toxicity, Anti-inflammatory, Hepatoprotective and in vitro Antioxidant Activities

R. A. El Dib

¹Department of Pharmacognosy, College of Pharmacy (Girls), King Saud University, Riyadh, Saudi Arabia

²Department of Pharmacognosy, College of Pharmacy, Helwan University, Cairo, Egypt

,

A. H. Gaara

³Department of Chemistry of Natural Compounds, National Research Center, Cairo, Egypt

,

S. M. El-Shenawy

⁴Department of Pharmacology, National Research Center, Cairo, Egypt

,

J. A. A. Micky

⁵Department of Chemistry, Faculty of Science, Alazhar University, Cairo, Egypt

,

A. A. Mohammed

⁴Department of Pharmacology, National Research Center, Cairo, Egypt

,

M. S. Marzouk

⁶Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia

⁷Chemistry of Natural Products Group, Center of Excellence for Advanced Sciences National Research Center, Cairo, Egypt

› Author Affiliations

Abstract

10 polyphenols were identified from 80% aqueous methanol extract (AME) of Markhamia platycalyx [(Baker) Sprague] leaf. Their structures were characterized as protocatechuic acid (1), E-caffeic acid (2), E-methyl caffeate (3), isoverbascoside (4), verbascoside (5), jacraninoside-I (6), cosmosiin (7), cinaroside (8), luteolin (9) and apigenin (10) based on chemical and extensive spectral studies (UV, ESI-MS, ¹H, ¹³C and 1D/2D NMR). Biological evaluation indicated that AME is non-toxic to the experimental animals. It exhibited a significant inhibition of oedema after 1, 2, 3 and 4 h for all examined doses (250, 500 and 1 000 mg/kg). In comparison with silymarin, the AME demonstrated a significant hepatoprotective effect in the form of high reduction in elevated ALT and AST serum levels in regard to paracetamol treated group. As well as, it revealed a marked significant scavenging activity by 62.9, 82.5, 88.3, 83.7 and 83.7%, for the concentrations 20, 40, 60, 80 and 100 mg/ml of the extract, respectively, relative to L-ascorbic acid (86.8%), which was used as a reference antioxidant drug.

Key words

Bignoniaceae - Markhamia platycalyx leaf - acute toxicity - hepatoprotection - anti-inflamatory - in vitro antioxidant

Full Text

References

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