Correlation among the BRAF Gene Mutation Status, Clinicopathological Features of Primary Tumour, and Lymph Node Metastasizing of Papillary Thyroid Carcinoma

J. Lukas; J. Drabek; B. Dudesek; P. Vazan; J. Stranska; S. Jancik; M. Mackova; M. Syrucek; D. Lukas; J. Duskova; P. Dundr; B. Hintnausova; J. Jiskra

doi:10.1055/s-0034-1372624

Experimental and Clinical Endocrinology & Diabetes, Table of Contents

Exp Clin Endocrinol Diabetes 2014; 122(05): 268-272
DOI: 10.1055/s-0034-1372624

Article

Correlation among the BRAF Gene Mutation Status, Clinicopathological Features of Primary Tumour, and Lymph Node Metastasizing of Papillary Thyroid Carcinoma

J. Lukas^*

¹Department of Otolaryngology – Head and Neck Surgery, Na Homolce Hospital, Prague, Czech Republic

²Department of Otolaryngology, Faculty of Medicine, Charles University, Pilsen, Czech Republic

,

J. Drabek^*

³Laboratory of Experimental Medicine, the Institute of Molecular and Translational Medicine of the Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic

,

B. Dudesek

⁴Department of Surgery, Atlas Hospital, Zlín, Czech Republic

,

P. Vazan

⁵Biopsy and Cytology Laboratory of J.A.Baťa, Zlín, Czech Republic

,

J. Stranska

³Laboratory of Experimental Medicine, the Institute of Molecular and Translational Medicine of the Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic

,

S. Jancik

³Laboratory of Experimental Medicine, the Institute of Molecular and Translational Medicine of the Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic

,

M. Mackova

⁶Department of Nuclear Medicine and Endocrinology, 2nd Medical Faculty, Charles University, Prague, Czech Republic

,

M. Syrucek

⁷Department of Pathology, Na Homolce Hospital, Prague, Czech Republic

,

D. Lukas

⁸Surgery Clinics, 3rd Medical Faculty, Charles University and Faculty Hospital Královské Vinohrady, Prague, Czech Republic

,

J. Duskova

⁹Institute of Pathology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

,

P. Dundr

⁹Institute of Pathology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

,

B. Hintnausova

¹⁰Department of Endocrinology, Na Homolce Hospital, Prague, Czech Republic

,

J. Jiskra

¹¹The 3rd Department of Medicine – Department of Endocrinology and Metabolism 1st Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Czech Republic

› Author Affiliations

Abstract

Background: Papillary thyroid carcinoma (PTC) is the most common malignant thyroid tumour. A common mutation of papillary thyroid carcinoma (PTC) is the somatic mutation of the BRAF ^V600E gene.

Aim: The aim was to 1) determine the association of lymph node metastases of PTC with the BRAF gene mutation of primary tumour; 2) evaluate association of the BRAF mutation in the primary tumour with clinicopathological parameters; 3) examine the extent of genetic heterogeneity by monitoring the BRAF mutation in multicentric tumours.

Subjects and methods: Retrospective analysis of the BRAF ^V600E mutation in PTC and PTC neck lymph node metastases in 156 patients operated from 2003 to 2012 in Prague and Zlín, the Czech Republic, using a qPCR assay. The results were correlated with clinicopathological factors.

Results: DNA was successfully extracted from 137 samples. The BRAF ^V600E mutation was detected in 78 cases (56.9%). The patients with BRAF p.Val600Glu mutation of primary tumour had only non-significantly higher risk of cervical lymph node metastases [OR=2.39 (95%) CI 1.00–5.75, p=0.052]. In the classic papillary variant, the BRAF ^V600E mutation was found significantly more often than in other PTC subtypes (p=0.022). We did not confirm any significant association between the BRAF ^V600E mutation and other clinicopathological findings.

Conclusion: Except for the higher prevalence in papillary variant of PTC, BRAF p.Val600Glu mutation was not associated with other prognostic clinicopathological factors of PTC. BRAF mutation cannot be regarded as a reliable marker of node metastases in patients with PTC.

Key words

papillary carcinoma - BRAF^V600E mutation - clinical and pathological parameters - neck node metastases

Full Text

References

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