Characterization of Extracellular Signal-Regulated Kinase 5 Levels in Human Umbilical Vein Endothelial Cells Exposed to Disturbed and Uniform Flow

Sherif Shalaby; Gautham Chitragari; Brandon J. Sumpio; Bauer E. Sumpio

doi:10.1055/s-0034-1378136

International Journal of Angiology, Table of Contents

Int J Angiol 2014; 23(03): 187-192
DOI: 10.1055/s-0034-1378136

Original Article

Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Characterization of Extracellular Signal-Regulated Kinase 5 Levels in Human Umbilical Vein Endothelial Cells Exposed to Disturbed and Uniform Flow

Sherif Shalaby

¹Department of Vascular Surgery, Yale University School of Medicine, New Haven, Connecticut

,

Gautham Chitragari

¹Department of Vascular Surgery, Yale University School of Medicine, New Haven, Connecticut

,

Brandon J. Sumpio

¹Department of Vascular Surgery, Yale University School of Medicine, New Haven, Connecticut

,

Bauer E. Sumpio

¹Department of Vascular Surgery, Yale University School of Medicine, New Haven, Connecticut

› Author Affiliations

Abstract

Extracellular signal-regulated kinase 5 (ERK5) has been reported to regulate endothelial cell integrity and protect from vascular dysfunction under continuous laminar flow. However, the effect of flow on ERK5 levels has not been determined. Confluent human umbilical vein endothelial cells (HUVECs) were seeded on fibronectin coated glass slides and serum starved for 2 hours with 1% fetal bovine serum (FBS). HUVECs were then exposed to to and fro flow (TFF), pulsatile forward flow (PFF), or continuous laminar flow (CLF) in a parallel plate flow chamber for up to 2 hours. At the end of experiment, cell lysates were prepared and immunoblotted with antibodies to total ERK5. Both CLF and TFF exhibited a decrease in ERK5 after levels after 2-hour exposure. However, the level of ERK5 for PFF remained the same. Disturbed, but not uniform pulsatile, flow decreases ERK5 levels in HUVECs.

Keywords

pulsatile uniform flow - atherosclerosis - HUVECs - ERK5 - disturbed flow - laminar flow - uniform flow

Full Text

References

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