Synthesis 2015; 47(19): 3061-3066
DOI: 10.1055/s-0034-1380922
paper
© Georg Thieme Verlag Stuttgart · New York

Concise Chemical Synthesis of the Tetrasaccharide Repeating Unit of the O-Antigen from Bovine Mastitis Isolate E. coli Serotype O174:H28

Ankita Mitra
Indian Institute of Science Education and Research Kolkata, Mohanpur 741246, India   eMail: sugarnet73@hotmail.com
,
Balaram Mukhopadhyay*
Indian Institute of Science Education and Research Kolkata, Mohanpur 741246, India   eMail: sugarnet73@hotmail.com
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Publikationsverlauf

Received: 13. Februar 2015

Accepted after revision: 30. April 2015

Publikationsdatum:
03. Juli 2015 (online)


Abstract

A concise strategy for the chemical synthesis of the tetrasaccharide repeating unit of the O-antigen from E. coli O174:H28 is reported. Synthesis of the target structure is accomplished by sequential glycosylation of rationally protected monosaccharide synthons derived from commercially available sugars. Glycosylation reactions were achieved by the activation of the thioglycosides using N-iodosuccinimide in the presence of H2SO4–silica. The carboxylic acid moiety of the glucuronic acid unit was successfully installed by a late TEMPO-mediated oxidation of the primary hydroxy group. Phthalimido groups were successfully used as the precursors of the required acetamido functionalities for 1,2-trans-glycosylations.

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