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Planta Med 2014; 80(18): 1678-1684
DOI: 10.1055/s-0034-1383195
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

In Vitro Anti-Inflammatory and Wound-Healing Potential of a Phyllostachys edulis Leaf Extract – Identification of Isoorientin as an Active Compound

Jonas Wedler
1   Institute for Pharma Technology, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Muttenz, Switzerland
,
Tony Daubitz
1   Institute for Pharma Technology, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Muttenz, Switzerland
,
Götz Schlotterbeck
2   Institute for Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Muttenz, Switzerland
,
Veronika Butterweck
1   Institute for Pharma Technology, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Muttenz, Switzerland
› Author Affiliations
Further Information

Publication History

received 15 July 2014
revised 18 September 2014

accepted 22 September 2014

Publication Date:
15 October 2014 (online)

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Abstract

Extracts prepared from the leaves of Phyllostachys edulis (bamboo) have received attention in pharmacological research due to their potent antitumor, anti-inflammatory, antimicrobial, and anti-ulcerogenic activities. In this study, anti-inflammatory effects of a bamboo leaf extract on tumor necrosis factor alpha-induced overproduction of interleukin 8, vascular endothelial growth factor, and interleukin 6 in immortalized human keratinocytes were investigated for the first time. In addition, wound-healing effects were evaluated in 3T3-swiss albino mouse fibroblasts. Bamboo leaf extract and isoorientin inhibited the tumor necrosis factor alpha-induced release of interleukin 8 and vascular endothelial growth factor. Furthermore, isoorientin dose-dependently reduced levels of interleukin 6 in tumor necrosis factor alpha-α-treated immortalized human keratinocytes cells. Wound healing was evaluated using a modification of the classical scratch assay. For evaluation of the wound gap, a new computerized method based on time-lapse microscopy was developed. It was shown that bamboo leaf extract (10 µg/mL) improved wound closure by 28 % (12 h) and 54 % (24 h), respectively. In concentrations of 50 µg/mL and above, bamboo leaf extract inhibited cell migration without affecting cell viability. Isoorientin (10 µM) improved wound closure by 29 % (12 h) and 56 % (24 h), respectively. Comparable to bamboo leaf extract, higher concentrations of isoorientin prevented cell migration. It is suggested that bamboo leaf extract as well as isoorientin have a dual activity – in higher doses, they show anti-inflammatory effects, and in lower concentrations, they exert anti-angiogenic activities.

Key words

Phyllostachys edulis - Poaceae - bamboo - wound healing - anti-inflammatory - time-lapse microscopy

Supporting Information

 

  • References

  • 1 Strodtbeek F. Physiology of wound healing. Newborn Infant Nurs Rev 2001; 1: 43-52
    CrossrefPubMedGoogle Scholar
  • 2 Singer AJ, Clark RAF. Mechanisms of disease: cutaneous wound healing. N Engl J Med 1999; 341: 738-746
    CrossrefPubMedGoogle Scholar
  • 3 Gurtner GC, Werner S, Barrandon Y, Langaker MT. Wound repair and regeneration. Nature 2008; 453: 314-321
    CrossrefPubMedGoogle Scholar
  • 4 Stenvinkel P, Ketteler M, Johnson RJ, Lindholm B, Pecoits-Filho R, Riella M, Heimburger O, Cederholm T, Girndt M. IL-10, IL-6, and TNF-alpha: central factors in the altered cytokine network of uremia–the good, the bad, and the ugly. Kidney Int 2005; 67: 1216-1233
    CrossrefPubMedGoogle Scholar
  • 5 Walz A, Peveri P, Aschauer H, Baggiolini M. Purification and amino-acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes. Biochem Bioph Res Commun 1987; 149: 755-761
    CrossrefPubMedGoogle Scholar
  • 6 Unemori EN, Amento EP, Bauer EA, Horuk R. Melanoma growth-stimulatory activity/GRO decreases collagen expression by human fibroblasts. Regulation by C–X-C but not C-C cytokines. J Biol Chem 1993; 268: 1338-1342
    PubMedGoogle Scholar
  • 7 de Vries C, Escobedo JA, Ueno H, Houck K, Ferrara N, Williams LT. The fms-like tyrosine kinase, a receptor for vascular endothelial growth factor. Science 1992; 255: 989-991
    CrossrefPubMedGoogle Scholar
  • 8 Heinlin J, Schreml S, Babilas P, Landthaler M, Karrer S. Cutaneous wound healing. Therapeutic interventions. Hautarzt 2010; 61: 611-626
    CrossrefPubMedGoogle Scholar
  • 9 Schreml S, Szeimies RM, Prantl L, Landthaler M, Babilas P. Wound healing in the 21st century. J Am Acad Dermatol 2010; 63: 866-881
    CrossrefPubMedGoogle Scholar
  • 10 Reuter J, Merfort I, Schempp CM. Botanicals in dermatology: an evidence-based review. Am J Clin Dermatol 2010; 11: 247-267
    PubMedGoogle Scholar
  • 11 Oprins J, Van Trier H. Bamboo, a material for landscape and garden design. Basel: Birkhäuser Publishers; 2006
    Google Scholar
  • 12 Higa JK, Liang Z, Williams PG, Panee J. Phyllostachys edulis compounds inhibit palmitic acid-induced monocyte chemoattractant protein 1 (MCP-1) production. PLoS One 2012; 7: e45082
    CrossrefPubMedGoogle Scholar
  • 13 Higa JK, Panee J. Bamboo extract reduces interleukin 6 (IL-6) overproduction under lipotoxic conditions through inhibiting the activation of NF-kappa B and AP-1 pathways. Cytokine 2011; 55: 18-23
    CrossrefPubMedGoogle Scholar
  • 14 Jung SH, Lee JM, Lee HJ, Kim CY, Lee EH, Um LH. Aldose reductase and advanced glycation endproducts inhibitory effect of Phyllostachys nigra . Biol Pharm Bull 2007; 30: 1569-1572
    CrossrefPubMedGoogle Scholar
  • 15 Kim SH, Kim TS, Kim SJ, Seong CN, Lee OH, Lee HJ, Yoo JC. Inhibition of interleukin-12 production in mouse macrophages via suppression of nuclear factor-kappa B binding activity by Phyllostachys nigra var. henonis . Immunopharmacol Immunotoxicol 2007; 29: 131-139
    CrossrefPubMedGoogle Scholar
  • 16 Kim SH, Kim TS, Lee HJ, Yoo JC. Enhancement of 1, 25-dihydroxyvitamin D-3- and all-trans retinoic acid-induced differentiation of human leukemia HL-60 cells by Phyllostachys nigra var. henonis . Immunopharmacol Immunotoxicol 2007; 29: 119-129
    CrossrefPubMedGoogle Scholar
  • 17 Shanmugasundaram OL, Gowda RVM. Development and characterization of bamboo gauze fabric coated with polymer and drug for wound healing. Fiber Polym 2011; 12: 15-20
    CrossrefPubMedGoogle Scholar
  • 18 Paquet P, Pierard GE. Interleukin-6 and the skin. Int Arch Allergy Immunol 1996; 109: 308-317
    CrossrefPubMedGoogle Scholar
  • 19 Boukamp P, Petrussevska RT, Breitkreutz D, Hornung J, Markham A, Fusenig NE. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 1988; 106: 761-771
    CrossrefPubMedGoogle Scholar
  • 20 Pastore S, Lulli D, Potapovich AI, Fidanza P, Kostyuk VA, Dellambra E, De Luca C, Maurelli R, Korkina LG. Differential modulation of stress-inflammation responses by plant polyphenols in cultured normal human keratinocytes and immortalized HaCaT cells. J Dermatol Sci 2011; 63: 104-114
    PubMedGoogle Scholar
  • 21 Kao ES, Hsu JD, Wang CJ, Yang SH, Cheng SY, Lee HJ. Polyphenols extracted from Hibiscus sabdariffa L. inhibited lipopolysaccharide-induced inflammation by improving antioxidative conditions and regulating cyclooxygenase-2 expression. Biosci Biotechnol Biochem 2009; 73: 385-390
    CrossrefPubMedGoogle Scholar
  • 22 Kim HJ, Xu L, Chang KC, Shin SC, Chung JI, Kang D, Kim SH, Hur JA, Choi TH, Kim S, Choi J. Anti-inflammatory effects of anthocyanins from black soybean seed coat on the keratinocytes and ischemia-reperfusion injury in rat skin flaps. Microsurgery 2012; 32: 563-570
    CrossrefPubMedGoogle Scholar
  • 23 Park K, Lee JH, Cho HC, Cho SY, Cho JW. Down-regulation of IL-6, IL-8, TNF-alpha and IL-1 beta by glucosamine in HaCaT cells, but not in the presence of TNF-alpha. Oncol Lett 2010; 1: 289-292
    PubMedGoogle Scholar
  • 24 Park YM, Noh SU, Kim HS, Kang H, Lee JY, Kim HO. Epigallocatechin-3-gallate has an anti-inflammatory effect by regulating macrophage migration inhibitory factor-induced t helper related chemokines and cytokines in human HaCaT cells. Clin Immunol 2010; 135 (Suppl.) S124-S125
    PubMedGoogle Scholar
  • 25 Conforti F, Rigano D, Menichini F, Loizzo MR, Senatore F. Protection against neurodegenerative diseases of Iris pseudopumila extracts and their constituents. Fitoterapia 2009; 80: 62-67
    CrossrefPubMedGoogle Scholar
  • 26 Kupeli E, Aslan M, Gurbuz I, Yesilada E. Evaluation of in vivo biological activity profile of isoorientin. Z Naturforsch C 2004; 59: 787-790
    PubMedGoogle Scholar
  • 27 Yuan L, Wang J, Xiao HF, Wu WQ, Wang YT, Liu XB. MAPK signaling pathways regulate mitochondrial-mediated apoptosis induced by isoorientin in human hepatoblastoma cancer cells. Food Chem Toxicol 2013; 53: 62-68
    CrossrefPubMedGoogle Scholar
  • 28 Takematsu H, Tagami H. Mode of release of interleukin-8 from proliferating human epidermal keratinocytes in vitro . Exp Dermatol 1993; 2: 121-124
    CrossrefPubMedGoogle Scholar
  • 29 Viac J, Palacio S, Schmitt D, Claudy A. Expression of vascular endothelial growth factor in normal epidermis, epithelial tumors and cultured keratinocytes. Arch Dermatol Res 1997; 289: 158-163
    CrossrefPubMedGoogle Scholar
  • 30 Frank S, Hubner G, Breier G, Longaker MT, Greenhalgh DG, Werner S. Regulation of vascular endothelial growth factor expression in cultured keratinocytes. Implications for normal and impaired wound healing. J Biol Chem 1995; 270: 12607-12613
    CrossrefPubMedGoogle Scholar
  • 31 Larsen CG, Anderson AO, Oppenheim JJ, Matsushima K. Production of interleukin-8 by human dermal fibroblasts and keratinocytes in response to interleukin-1 or tumour necrosis factor. Immunology 1989; 68: 31-36
    PubMedGoogle Scholar
  • 32 Pietrzak AT, Zalewska A, Chodorowska G, Krasowska D, Michalak-Stoma A, Nockowski P, Osemlak P, Paszkowski T, Rolinski JM. Cytokines and anticytokines in psoriasis. Clin Chim Acta 2008; 394: 7-21
    CrossrefPubMedGoogle Scholar
  • 33 Smith JR, Lanier VB, Braziel RM, Falkenhagen KM, White C, Rosenbaum JT. Expression of vascular endothelial growth factor and its receptors in rosacea. Br J Ophthalmol 2007; 91: 226-229
    CrossrefPubMedGoogle Scholar
  • 34 Xia YP, Li B, Hylton D, Detmar M, Yancopoulos GD, Rudge JS. Transgenic delivery of VEGF to mouse skin leads to an inflammatory condition resembling human psoriasis. Blood 2003; 102: 161-168
    CrossrefPubMedGoogle Scholar
  • 35 Choi YJ, Lim HS, Choi JS, Shin SY, Bae JY, Kang SW, Kang IJ, Kang YH. Blockade of chronic high glucose-induced endothelial apoptosis by Sasa borealis bamboo extract. Exp Biol Med 2008; 233: 580-591
    CrossrefPubMedGoogle Scholar
  • 36 Werner S, Krieg T, Smola H. Keratinocyte-fibroblast interactions in wound healing. J Invest Dermatol 2007; 127: 998-1008
    CrossrefPubMedGoogle Scholar
  • 37 Bainbridge P. Wound healing and the role of fibroblasts. J Wound Care 2013; 22: 407-408 410–412
    CrossrefPubMedGoogle Scholar
  • 38 Baum CL, Arpey CJ. Normal cutaneous wound healing: clinical correlation with cellular and molecular events. Dermatol Surg 2005; 31: 674-686
    CrossrefPubMedGoogle Scholar