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Planta Med 2015; 81(01): 71-78
DOI: 10.1055/s-0034-1383312
Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

HPLC Quantification of All Five Ginkgolic Acid Derivatives in Ginkgo biloba Extracts using 13 : 0 Ginkgolic Acid as a Single Marker Compound

Ruwei Wang
1   Zhejiang CONBA Pharmaceutical & Drug Research Development Corporation, Hangzhou, China
2   Zhejiang Key Laboratory for Traditional Chinese Medicine, Pharmaceutical Technology, Zhejiang, China
,
Yuta Kobayashi
3   Faculty of Medicine, Shimane University, Izumo, Japan
,
Yu Lin
4   Medical Corporation Soujikai, Osaka, Japan
,
Hans Wilhelm Rauwald
5   Department of Pharmaceutical Biology, Leipzig University, Leipzig, Germany
,
Jianbiao Yao
1   Zhejiang CONBA Pharmaceutical & Drug Research Development Corporation, Hangzhou, China
2   Zhejiang Key Laboratory for Traditional Chinese Medicine, Pharmaceutical Technology, Zhejiang, China
,
Ling Fang
1   Zhejiang CONBA Pharmaceutical & Drug Research Development Corporation, Hangzhou, China
2   Zhejiang Key Laboratory for Traditional Chinese Medicine, Pharmaceutical Technology, Zhejiang, China
,
Hongxiang Qiao
1   Zhejiang CONBA Pharmaceutical & Drug Research Development Corporation, Hangzhou, China
2   Zhejiang Key Laboratory for Traditional Chinese Medicine, Pharmaceutical Technology, Zhejiang, China
,
Kenny Kuchta
5   Department of Pharmaceutical Biology, Leipzig University, Leipzig, Germany
6   Natural Products Chemistry Research, Department of Food and Nutrition, Sanyo Gakuen University, Okayama, Japan
› Author Affiliations
Further Information

Publication History

received 18 March 2014
revised 11 October 2014

accepted 23 October 2014

Publication Date:
17 December 2014 (online)

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Abstract

An HPLC quantification method for ginkgolic acid derivatives in Ginkgo biloba leaf extracts was developed. Using 13 : 0 ginkgolic acid as a marker compound, the relative correlation factors of the four other ginkgolic acid derivatives – namely, 15 : 0 ginkgolic acid, 15 : 1 ginkgolic acid, 17 : 1 ginkgolic acid, and 17 : 2 ginkgolic acid – to 13 : 0 ginkgolic acid were determined by HPLC and subsequently used for calculating their contents in ten hydro-ethanolic refined extract samples. In other words, the content of 13 : 0 ginkgolic acid in the extracts was determined using the isolated compound as an external standard. Subsequently the now known concentration of this compound functioned as an internal standard for the quantification of the other four ginkgolic acid derivatives via the described correlation factors. This HPLC method was validated by two independent control measurements, one with an external standard for every individual compound and one based on the present method with the single marker compound alone. The results did not differ significantly in any of the 10 tested extract samples. The protocol presented here thus not only uses the same reference substance for G. biloba extracts as the current Chinese Pharmacopoeia method but also incorporates the advantages of the current European Pharmacopoeia approach. It is simple, reproducible, and can be used to determine the total contents of ginkgolic acid derivatives in G. biloba leaf extracts.

Key words

Ginkgo biloba - Ginkgoaceae - ginkgolic acid derivatives - relative correlation factor - HPLC - quantitative analysis

Supporting Information

 

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