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DOI: 10.1055/s-0034-1384523
Real-Time Monitoring of GPCR/cAMP Signalling by FRET and Single-Molecule Microscopy
Publikationsverlauf
received 31. März 2014
accepted 17. Juni 2014
Publikationsdatum:
23. Juli 2014 (online)
![](https://www.thieme-connect.de/media/hmr/201412/lookinside/thumbnails/10.1055-s-0034-1384523-1.jpg)
Abstract
G-protein-coupled receptors (GPCRs), located on the surface of virtually every cell in our organism, mediate the effects of many hormones and neurotransmitters. Although GPCRs have been extensively studied for more than 4 decades using pharmacological and biochemical methods, the recent introduction of optical methods such as fluorescence resonance energy transfer (FRET) and single-molecule microscopy is fostering novel and important discoveries in the field. Here, we review the use of such optical methods, focusing on some recent examples of their application to important and still unresolved questions concerning the spatial organisation and dynamics of GPCR signalling.
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